In search for genetic factors controlling the sexualization in diatoms

• Project/Area Number: 15K18742
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Fukui Prefectural University
• Principal Investigator: SATO Shinya 福井県立大学, 海洋生物資源学部, 講師 (80709163)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 珪藻 / 生殖 / ゲノム / トランスクリプトーム / 細菌 / オルガネラ / 生活史 / ストレス

Outline of Final Research Achievements

This project was undertaken in order to reveal the genetic factors controlling the sexualization and sex determination in diatoms with Pseudostaurosira trainorii whose sex is readily controllable in a laboratory condition. Sequence analyses and comparative transcriptome analyses are currently ongoing for sex-specific region in its genome. Complete genome sequences were also obtained for mitochondrion and chloroplast: the latter was the largest in genome size among the diatom cp genome sequenced so far, and showed an evidence for a contribution of lateral gene transfer for its size expansion. Crossing experiment revealed uniparental inheritance of both organellar genome in this species. This study also suggested a possibility of an involvement of Marinobacter bacteria during a process of sexualization in the diatom - this finding would pave the way to better understand the mechanism of sexualization in diatoms.

Research Products

• [Journal Article] A combined morphological and molecular approach to Nitzschia varelae sp. nov., with discussion of symmetry in Bacillariaceae (2017)
  • Author(s): Rafael Carballeira, Rosa Trobajo, Manel Leira, Xavier Benito, Shinya Sato and David G. Mann
  • Journal Title: European Journal of Phycology Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] New Insights into Plagiogrammaceae (Bacillariophyta) Based on Multigene Phylogenies and Morphological Characteristics with the Description of a New Genus and Three New Species (2015)
  • Author(s): Li CL, Ashworth MP, Witkowski A, Dabek P, Medlin LK, Kooistra WHCF, Sato S, Zglobicka I, Kurzydlowski KJ, Theriot EC, S.M. Sabir JSM, Khiyami MA, Mutwakil MHZ, Sabir MJ, Alharbi NS, Hajarah NH, Qing S, Jansen RK
  • Journal Title: PLoS ONE Volume: 10 Issue: 10 Pages: 1-26
  • DOI: 10.1371/journal.pone.0139300
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic and Physiological Diversity in the Diatom Nitzschia inconspicua (2015)
  • Author(s): Rovira R, Trobajo R, Sato S, Ibanez C & DG Mann
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 62 Issue: 6 Pages: 815-832
  • DOI: 10.1111/jeu.12240
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Characterization of Gambierdiscus and Coolia (Dinophyceae) isolates from Thailand based on morphology and phylogeny (2015)
  • Author(s): Tawong W, Nishimura T, Sakanari H, Sato S, Yamaguchi H and Adachi M
  • Journal Title: Phycological Research Volume: 63 Issue: 2 Pages: 125-133
  • DOI: 10.1111/pre.12074
  • Peer Reviewed
• [Presentation] パルマ藻株Triparma retinervisの確立により示された珪質型パルマ藻の非単系統性 (2017)
  • Author(s): 山田和正, 山崎誠司, 佐藤晋也, 吉川伸哉, 桑田晃, 一宮睦雄
  • Organizer: 日本藻類学会第41回大会
  • Place of Presentation: 高知大学
  • Year and Date: 2017-03-24
• [Presentation] 汽水産珪藻Pseudostaurosiraの被殻を裏打ちする有機層の構造的，生化学的特徴 (2017)
  • Author(s): 中村憲章, 真山茂樹, 佐藤晋也
  • Organizer: 日本藻類学会第41回大会
  • Place of Presentation: 高知大学
  • Year and Date: 2017-03-24
• [Presentation] Comparison of genome between opposite mating types of Pseudostaurosira trainorii. (2017)
  • Author(s): Nakamura N, Sato S
  • Organizer: 11th International Phycological Congress
  • Int'l Joint Research
• [Presentation] Pseudostaurosira trainoriiにおけるオルガネラの遺伝様式の解明 (2017)
  • Author(s): 中村憲章，礒野弘之，佐藤晋也
  • Organizer: 珪藻学会第37回研究集会
• [Presentation] パルマ藻Triparma laevisのプロトプラストを利用した珪酸質殻形成過程の解析 (2016)
  • Author(s): 山田和正, 桑田晃, 長里千香子, 本村泰三, 桂大貴, 佐藤晋也, 吉川伸哉, 一宮睦雄
  • Organizer: 2016年度日本ベントス学会・日本プランクトン学会合同大会
  • Place of Presentation: 熊本県立大学
  • Year and Date: 2016-09-07
• [Presentation] Valve morphogenesis in a multipolar diatom genus Hydrosera (2016)
  • Author(s): Idei M, Osada K, Sato S, Nagumo T, Mann DG
  • Organizer: International Diatom Symposium
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Variation in morphological characters of diatotepum in relation to valve ultrastructure (2016)
  • Author(s): Nakamura N, Sato S, Julius ML, Maeda Y, Tanaka T, Fujimoto K, Mayama S
  • Organizer: International Diatom Symposium
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] 珪藻のガラス質細胞壁を裏打ちする有機層diatotepumの構造的多様性 (2016)
  • Author(s): 中村憲章, 佐藤晋也, 田中剛, 前田義昌, 藤本光一郎, 真山茂樹
  • Organizer: 北陸植物学会平成２８年度大会
  • Place of Presentation: 富山大学
  • Year and Date: 2016-06-10
• [Presentation] Genetic, morphological, and ecophysiological diversity in Nitzschia inconspicua (Bacillariophyta) and implications for ecological assessment of waterbodies. (2016)
  • Author(s): Rovira L, Trobajo R, Sato S, Kelly M, Ibanez C, Krokowski J, Mann DG
  • Organizer: British Phycological Society Annual Meeting
  • Place of Presentation: Bornemouth, UK.
  • Year and Date: 2016-06-02
  • Int'l Joint Research
• [Presentation] 珪藻の珪酸質細胞壁を裏打ちする有機層の構造的特性 (2016)
  • Author(s): 中村憲章, 佐藤晋也, 田中剛, 前田義昌, 藤本光一郎, 真山茂樹
  • Organizer: 28回日本水産学会中部支部大会
  • Place of Presentation: 福井県立大学
• [Presentation] Sexual reproduction of diatom: evidence for bacterial involvement. (2015)
  • Author(s): Sato S, Takao Y
  • Organizer: 第二回分子珪藻研究会.
  • Place of Presentation: 関西学院大学大阪梅田キャンパス. 大阪.
  • Year and Date: 2015-11-28
  • Int'l Joint Research
• [Presentation] 細菌と珪藻生殖. (2015)
  • Author(s): 佐藤晋也
  • Organizer: 珪藻学会第35回研究集会.
  • Place of Presentation: 日光交流促進センター. 栃木.
  • Year and Date: 2015-11-07
• [Presentation] Hydrosera 属の殻内面を被う新奇鱗片状プレートについて. (2015)
  • Author(s): 出井雅彦, 長田敬吾, 佐藤晋也, 南雲保
  • Organizer: 珪藻学会第35回研究集会.
  • Place of Presentation: 日光交流促進センター. 栃木.
  • Year and Date: 2015-11-07
• [Presentation] パルマ藻Triparma laevis の細胞外皮形成過程の微細構造解析. (2015)
  • Author(s): 山田和正, 桂大貴, 佐藤晋也, 神谷充伸, 大城香, 一宮睦雄, 桑田晃, 長里千香子, 本村泰三, 吉川伸哉
  • Organizer: 日本植物学会第79回大会.
  • Place of Presentation: 新潟コンベンションセンター. 新潟.
  • Year and Date: 2015-09-06
• [Presentation] An implication of bacteria-diatom interaction at the onset of sexual reproduction in diatoms. (2015)
  • Author(s): Sato S
  • Organizer: 6th European Phycological Congress.
  • Place of Presentation: London, UK.
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] A molecular approach to Nitzschia: establishing a framework for classifying a highly diverse and taxonomically difficult diatom group. (2015)
  • Author(s): Trobajo R, Mann DG, Li C, Rovira L, Sato S, Dabek P, Witkowski A
  • Organizer: 6th European Phycological Congress.
  • Place of Presentation: London, UK.
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Valve and scale-like plate morphogenesis in a multipolar diatom genus Hydrosera. 6th European Phycological Congress. (2015)
  • Author(s): Idei M, Osada K, Sato S, Nagumo T, Mann DG
  • Organizer: 6th European Phycological Congress.
  • Place of Presentation: London, UK.
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] パルマ藻から珪藻の進化の秘密を探る. (2015)
  • Author(s): 桑田晃, 佐藤晋也
  • Organizer: 日本進化学会17回大会. ワークショップ 「珪藻の進化・繁栄の謎を握る道の藻類：パルマ藻の生物学」.
  • Place of Presentation: 中央大学後楽園キャンパス. 東京.
  • Year and Date: 2015-08-20
• [Presentation] Valve formation in araphid diatoms. (2015)
  • Author(s): Tiffany MA and Sato S
  • Organizer: Molecular Life of Diatoms 2015.
  • Place of Presentation: Seattle, USA.
  • Year and Date: 2015-07-07
  • Int'l Joint Research
• [Presentation] 珪藻の種と分子系統. (2015)
  • Author(s): 佐藤晋也
  • Organizer: 日本珪藻学会第36回大会. シンポジウム 「珪藻の種とは？」.
  • Place of Presentation: 東京大学弥生キャンパス. 東京.
  • Year and Date: 2015-05-09
  • Invited
• [Presentation] パルマ藻のプレート形成. (2015)
  • Author(s): 山田和正, 桂大貴, 佐藤晋也, 長里千香子, 本村泰三, 一宮睦雄, 桑田晃, 吉川伸哉
  • Organizer: 日本珪藻学会第36回大会.
  • Place of Presentation: 東京大学弥生キャンパス. 東京.
  • Year and Date: 2015-05-09
• [Book] みんなが知りたい海藻の疑問50 (2016)
  • Author(s): 田中次郎（編）、佐藤晋也（分担執筆）
  • Publisher: 成山堂書店