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Environmental stress response of biosynthetic pathway of osmoprotectants in marine microorganisms.

Research Project

Project/Area Number 15K18743
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionMeijo University

Principal Investigator

Hakuto Kageyama  名城大学, 理工学部, 准教授 (40444369)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords環境ストレス / シアノバクテリア / 浸透圧適合溶質 / 海洋性藻類 / 環境ストレス応答
Outline of Final Research Achievements

To clarify the mechanisms of salt tolerance in cyanobacteria and marine microalgae, we analyzed them at molecular level. As a result, novel findings below were observed. (1) Candidate enzyme for DMSP synthetic pathway in marine microalgae was obtained. (2) Nitrate oversupply affected glycine betaine and mycosporine-2-glycine synthesis in response to changes of salinity in a halotolerant cyanobacterium. (3) DNA damage protecting and free radical scavenging properties of mycosporine-2-glycine were observed. (4) Overexpression of halophilic serine hydroxymethyltransferase in fresh water cyanobacterium results in enhanced salinity tolerance. etc.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 8 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results) Book (1 results)

  • [Int'l Joint Research] Chulalongkorn University(タイ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Overexpression of halophilic serine hydroxymethyltransferase in fresh water cyanobacterium Synechococcus elongatus PCC7942 results in increased enzyme activities of serine biosynthetic pathways and enhanced salinity tolerance2017

    • Author(s)
      Rungaroon Waditee-Sirisattha, Hakuto Kageyama, Yoshito Tanaka, Minoru Fukaya, and Teruhiro Takabe
    • Journal Title

      Archives of Microbiology

      Volume: 199 Issue: 1 Pages: 19-25

    • DOI

      10.1007/s00203-016-1271-z

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] DNA damage protecting and free radical scavenging properties of mycosporine-2-glycine from the Dead Sea cyanobacterium in A375 human melanoma cell lines2016

    • Author(s)
      Vipaporn Cheewinthamrongrod, Hakuto Kageyama, Tanapat Palaga, Teruhiro Takabe, Rungaroon Waditee-Sirisattha
    • Journal Title

      Journal of Photochemistry & Photobiology, B: Biology

      Volume: 164 Pages: 289-295

    • DOI

      10.1016/j.jphotobiol.2016.09.037

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Halophilic microorganisms resources and its application in industrial and environmental biotechnology.2016

    • Author(s)
      Rungaroon Waditee-Sirisattha, Hakuto Kageyama, and Teruhiro Takabe
    • Journal Title

      AIMS Microbiology

      Volume: 2 Issue: 1 Pages: 42-54

    • DOI

      10.3934/microbiol.2016.1.42

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Improved Alkane Production in Nitrogen Fixing and Halotolerant Cyanobacteria via Abiotic Stresses and Genetic Manipulation of Alkane Synthetic Genes2015

    • Author(s)
      Hakuto Kageyama, Rungaroon Waditee-Sirisattha, Sophon Sirisattha, Yoshito Tanaka, Aparat Mahakhant, and Teruhiro Takabe
    • Journal Title

      Current Microbiology

      Volume: 71 Issue: 1 Pages: 230-235

    • DOI

      10.1007/s00284-015-0833-7

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Caleosin from Chlorella vulgaris TISTR 8580 is salt-induced and heme-containing protein2015

    • Author(s)
      Pairpilin Charuchinda, Rungaroon Waditee-Sirisattha, Hakuto Kageyama, Daisuke Yamada, Sophon Sirisattha, Yoshito Tanaka, Aparat Mahakhant, and Teruhiro Takabe
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 79 Issue: 7 Pages: 1119-1124

    • DOI

      10.1080/09168451.2015.1010480

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Expression of developmentally regulated plasma membrane polypeptide (DREPP2) in rice root tip and interaction with Ca2+/CaM complex and microtubule2015

    • Author(s)
      Nana Yamada, Cattarin Theerawitaya, Hakuto Kageyama, Suriyan Cha-um, and Teruhiro Takabe
    • Journal Title

      Protoplasma

      Volume: 252 Issue: 6 Pages: 1519-1527

    • DOI

      10.1007/s00709-015-0781-x

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Nitrate and amino acid availability affects glycine betaine and mycosporine-2-glycine in response to changes of salinity in a halotolerant cyanobacterium Aphanothece halophytica2015

    • Author(s)
      Rungaroon Waditee-Sirisattha, Hakuto Kageyama, Minoru Fukaya, Vandna Rai, and Teruhiro Takabe
    • Journal Title

      FEMS Microbiology Letters

      Volume: 362 Issue: 23 Pages: fnv198-fnv198

    • DOI

      10.1093/femsle/fnv198

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Extraction and Quantification of Alkanes in Cyanobacteria2015

    • Author(s)
      Hakuto Kageyama, Rungaroon Waditee-Sirisattha, Sophon Sirisattha, Yoshito Tanaka, Aparat Mahakhant, and Teruhiro Takabe
    • Journal Title

      Bio-protocol

      Volume: 5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Further progress on the research of improved alkane production in cyanobacteria by abiotic stress2016

    • Author(s)
      Hakuto Kageyama
    • Organizer
      Japan-Thailand Collaboration Symposium (Meijo University and TISTR) The 4th Symposium of Perspective Innovation and Achievement of Algal Biofuel R&D (4th PIAB)
    • Place of Presentation
      Thailand Institute of Scientific & Technological Research (TISTR), Pathum Thani, Thailand
    • Related Report
      2016 Annual Research Report
  • [Presentation] Isolation and functional characterization of caleosin gene from the green microalgae Chlorella vulgaris2015

    • Author(s)
      Hakuto Kageyama, Rungaroon Waditee-Sirisattha, Sophon Sirisattha, Yoshito Tanaka, Aparat Mahakhant, and Teruhiro Takabe
    • Organizer
      The 27th Annual Meeting of the Thai Society for Biotechnology and International Conference, INNOVATIVE BIOTECHNOLOGY (TSB2015)
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Alkane Production in Nitrogen-Fixing and Halotolerant Cyanobacteria2015

    • Author(s)
      Rungaroon Waditee-Sirisattha, Hakuto Kageyama, Sophon Sirisattha, Pairpilin Charuchinda, Aparat Mahakhant, and Teruhiro Takabe
    • Organizer
      The 27th Annual Meeting of the Thai Society for Biotechnology and International Conference, INNOVATIVE BIOTECHNOLOGY (TSB2015)
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Book] Algal Green Chemistry: Recent Progress in Biotechnology2017

    • Author(s)
      Hakuto Kageyama, Rungaroon Waditee-Sirisattha, Yoshito Tanaka, and Teruhiro Takabe
    • Total Pages
      336
    • Publisher
      Elsevier
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2022-02-16  

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