Application of RNA interference in Screlactinian corals.

• Project/Area Number: 15K18744
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: University of Tsukuba (2016-2017); National Institute of Genetics (2015)
• Principal Investigator: YUYAMA Ikuko 筑波大学, 生命環境系, 特任助教 (80565995)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 造礁性サンゴ / 遺伝子ノックダウン / 細胞内共生 / RNAi / サンゴ / 骨格形成 / 褐虫藻 / GFP / Thioredoxin

Outline of Final Research Achievements

To date, only few studies have used RNA interference technology to Scleractinian corals. The aimed of this research is optimization and establishment of RNAi interference mediated gene knockdown in Scleractinian corals. The results demonstrated that RNAi causes down-regulation of expression of target genes and indicated that RNAi is an effective method for screening for gene function involved in coral-algal endosymbiosis and coral stress response.

Research Products

• [Journal Article] ゲノム，トランスクリプトームデータから明らかにされる刺胞動物-藻類の細胞内共生 (2018)
  • Author(s): 湯山育子
  • Journal Title: 生物科学 Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Temperature dependence of aragonite and calcite skeleton formation by a scleractinian coral in low mMg/Ca seawater (2017)
  • Author(s): Higuchi Tomihiko、Shirai Kotaro、Mezaki Takuma、Yuyama Ikuko
  • Journal Title: Geology Volume: 45 Issue: 12 Pages: 1087-1090
  • DOI: 10.1130/g39516.1
  • NAID: 120007127785
  • Peer Reviewed / Open Access
• [Journal Article] Symbiodinium kawagutii (clade F) coats the surface of Acropora solitaryensis, resulting in the formation of a sheet-like crust. (2016)
  • Author(s): Ikuko Yuyama, Tomihiko Higuchi, Takuma Mezaki
  • Journal Title: The Proceedings of the 13th International Coral Reef Symposium Volume: 1 Pages: 49-56
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sulfur utilization of corals is enhanced by endosymbiotic algae. (2016)
  • Author(s): Ikuko Yuyama, Tomihiko Higuchi, Yoshio Takei
  • Journal Title: Biology Open Volume: 5 Issue: 9 Pages: 1299-1304
  • DOI: 10.1242/bio.020164
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Different endosymbiotic interactions in two hydra species reflect the evolutionary history of endosymbiosis (2016)
  • Author(s): Masakazu Ishikawa, Ikuko Yuyama, Hiroshi Shimizu, Masafumi Nozawa, Kazuho Ikeo, Takashi Gojobori,
  • Journal Title: Genome Biology and Evolution Volume: 8 Issue: 7 Pages: 2155-2163
  • DOI: 10.1093/gbe/evw142
  • Peer Reviewed / Open Access
• [Journal Article] Different stress tolerances of juvenile polyps of the coral Acropora tenuis associated with clades C1 and D Symbiodinium. (2016)
  • Author(s): Ikuko Yuyama, Takashi Nakamura, Tomihiko Higuchi, Michio Hidaka
  • Journal Title: Zoological studies Volume: 55 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Different stresss tolerances of juvenile polyps of the coral Acropora tenuis associated with clades C1 and D Symbiodinium. (2016)
  • Author(s): Ikuko Yuyama, Takashi Nakamura, Tomihiko Higuchi, Michio Hidaka
  • Journal Title: Zoological studies Volume: 55
  • Peer Reviewed / Open Access
• [Journal Article] The northern limit of corals of the genus Acropora in temperate zone is determined by their resilience to cold bleaching. (2015)
  • Author(s): Tomihiko Higuchi, Sylvain Agostini, Beatriz Estela Casareto, Yoshimi Suzuki, Ikuko Yuyama
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 1-9
  • DOI: 10.1038/srep18467
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The combined effects of nitrate with high temperature and high light intensity on coral bleaching and antioxidant enzyme activities (2015)
  • Author(s): Tomihiko Higuchi, Ikuko Yuyama, Takashi Nakamura
  • Journal Title: Regional Studies in Marine Science Volume: 2 Pages: 27-31
  • DOI: 10.1016/j.rsma.2015.08.012
  • Peer Reviewed
• [Presentation] 造礁性サンゴー褐虫藻の細胞内共生に関与する研究 (2017)
  • Author(s): 湯山育子
  • Organizer: 第20回日本サンゴ礁学会
  • Invited
• [Presentation] 湯山育子 (2017)
  • Author(s): サンゴー褐虫藻の細胞内共生関係の破綻にはサンゴ免疫系の暴走が関係する
  • Organizer: 第50回日本原生生物学会大会-第1回日本共生生物学会大会
  • Invited
• [Presentation] Identification of genes related to aragonite/calcite crystal growth in corals (2017)
  • Author(s): Ikuko Yuyama, Tomihiko Higuchi
  • Organizer: The 14th International Symposium on Biomineralization (BIOMIN XIV)
  • Int'l Joint Research / Invited
• [Presentation] サンゴー褐虫藻の細胞内共生成立時に見られるダイナミックな遺伝子発現変動 (2017)
  • Author(s): 湯山育子
  • Organizer: 第19回マリンバイオテクノロジー学会大会
  • Invited
• [Presentation] RNA-seq解析により明らかにする細胞内共生性渦鞭毛藻のストレス応答 (2017)
  • Author(s): 湯山育子, 池尾一穂
  • Organizer: 日本藻類学会
  • Place of Presentation: 高知大学（高知県・高知市）
• [Presentation] DYNAMIC CHANGES IN GENE EXPRESSION DURING EARLY STAGE OF CORAL-ALGAL SYMBIOSIS (2016)
  • Author(s): Ikuko Yuyama, Kazuho Ikeo
  • Organizer: 13th International Coral Reef Symposium
  • Place of Presentation: Hawaii Convention Center
  • Year and Date: 2016-06-19
  • Int'l Joint Research
• [Presentation] Differential stress response of coral associating with clades C and D Symbiosis (2016)
  • Author(s): 湯山育子, 池尾一穂
  • Organizer: The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県・宜野湾市）
  • Int'l Joint Research
• [Presentation] DYNAMIC CHANGES IN GENE EXPRESSION DURING EARLY STAGE OF CORAL-ALGAL SYMBIOSIS (2016)
  • Author(s): Ikuko Yuyama, Kazuho Ikeo
  • Organizer: 13th International Coral Reef Symposium
  • Place of Presentation: ハワイコンベンションセンター（ホノルル・アメリカ合衆国）
  • Int'l Joint Research
• [Presentation] 造礁サンゴにおけるRNAi法の確立 (2015)
  • Author(s): 湯山育子、池尾一穂
  • Organizer: 第38回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] サンゴー褐虫藻の細胞内共生初期の分子応答から共生成立の謎を解く (2015)
  • Author(s): 湯山育子、池尾一穂
  • Organizer: 日本サンゴ礁学会第18回大会
  • Place of Presentation: 慶應義塾大学三田キャンパス
  • Year and Date: 2015-11-27
• [Presentation] サンゴー褐虫藻の細胞内共生成立時に見られるダイナミックな遺伝子発現変動 (2015)
  • Author(s): 湯山育子、野澤昌文、石川昌和、池尾一穂
  • Organizer: 第86回日本動物学会
  • Place of Presentation: 新潟市、朱鷺メッセ
  • Year and Date: 2015-09-17
• [Remarks] 筑波大学 研究者総覧
  • URL: http://www.trios.tsukuba.ac.jp/researcher/0000003962