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Visualization of ion movements in plants

Research Project

Project/Area Number 15K18761
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionThe University of Tokyo

Principal Investigator

Sugita Ryohei  東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (60724747)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsイメージング / RI / ライブイメージング / 元素輸送 / RRIS / RIイメージング / リアルタイムRIイメージング / 放射性同位元素 / シロイヌナズナ
Outline of Final Research Achievements

To understand ion movement in plants, we have been developing an live imaging system using radioisotopes. Each ion moves via xylem and phloem in plants. To understand ion transport mechanisms in plants, it is important to study xylem and phloem flow separately. So we tried to visualize only xylem flow using heat girdling treatment. The results show that magnesium was transported mainly via the xylem, while the phloem contribution is scarce. On the other hand, phosphate transport via the phloem was slower than via the xylem.
As a second step, we tried to visualize only xylem flow in rice in dark conditions. Magnesium and calcium transport are not influenced by light. On the other hand, phosphate transport in shoot was sensitively influenced by light. This results show that phosphate was transported via the xylem and phloem. On the other hand, the phloem contributions of magnesium, potassium, calcium, and cesium are scarce.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Visualization of how light changes affect ion movement in rice plants using a real-time radioisotope imaging system2017

    • Author(s)
      Sugita, R., Kobayashi, N.I., Hirose, Iwata, R., Suzuki, H., Tanoi, K., and Nakanishi, T.M.
    • Journal Title

      Journal of Radioanalytical and Nuclear Chemistry

      Volume: 印刷中 Issue: 3 Pages: 717-723

    • DOI

      10.1007/s10967-017-5193-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Visualization of Mineral Elements Uptake and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS)2016

    • Author(s)
      Sugita, R.; Kobayashi, N.I.; Hirose, A.; Saito, T.; Iwata, R.; Tanoi, K.; Nakanishi, T. M
    • Journal Title

      Plant and Cell Physiology

      Volume: 印刷中 Issue: 4 Pages: 743-753

    • DOI

      10.1093/pcp/pcw056

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 放射性同位元素を利用した植物体内イオンのライブイメージング2017

    • Author(s)
      杉田亮平、小林奈通子、廣瀬農、 田野井慶太朗、中西友子
    • Organizer
      第11回NIBBバイオイメージングフォーラム
    • Place of Presentation
      基礎生物学研究所(愛知県岡崎市)
    • Year and Date
      2017-02-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] RIによるライブイメージングと凍結切片イメージング2017

    • Author(s)
      杉田 亮平,広瀬 農,田野井 慶太朗
    • Organizer
      第5回植物イメージングの会
    • Place of Presentation
      日本女子大学(東京都文京区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] リアルタイムRIイメージングシステムを用いた植物体内元素動態の可視化技術の開発2016

    • Author(s)
      杉田亮平、小林奈通子、廣瀬農、 田野井慶太朗、中西友子
    • Organizer
      日本放射化学会
    • Place of Presentation
      新潟大学(新潟県新潟市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] シロイヌナズナにおける元素動態の可視化技術の開発2016

    • Author(s)
      杉田亮平、小林奈通子、廣瀬農、 田野井慶太朗、中西友子
    • Organizer
      第2回植物の栄養研究会
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] Visualization of Mineral Elements movement and the Dynamics of Photosynthates in Plants by a Newly Developed Real-Time Radioisotope Imaging System (RRIS)2016

    • Author(s)
      Sugita R.; Kobayashi N. I.; Hirose A.; Tanoi K.; Nakanishi T.M
    • Organizer
      The 17th International Workshop on Plant Membrane Biology (IWPMB2016)
    • Place of Presentation
      Maryland, USA
    • Year and Date
      2016-06-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization of the Uptake of the Elements and the Dynamics of Photosynthates Derived from 14CO2 in plants by Real-Time Radioisotope Imaging System.2016

    • Author(s)
      Ryohei Sugita, Natsuko I Kobayashi, Atsushi Hirose, Ren Iwata, Keitaro Tanoi, Tomoko M Nakanish
    • Organizer
      Radioanalytical and Nuclear Chemistry (RANC-2016)
    • Place of Presentation
      Hungary
    • Year and Date
      2016-04-10
    • Related Report
      2015 Research-status Report
  • [Presentation] 非破壊RIイメージングによる植物中元素動態の可視化2015

    • Author(s)
      杉田 亮平; 小林 奈通子; 廣瀬 農; 田野井 慶太朗; 中西 友子
    • Organizer
      日本土壌肥料学会2015年度京都大会
    • Place of Presentation
      京都大学
    • Year and Date
      2015-09-09
    • Related Report
      2015 Research-status Report
  • [Presentation] リアルタイムRIイメージングシステムを用いた植物根における元素輸送解析2015

    • Author(s)
      杉田 亮平; 小林 奈通子; 菅野 里美; 廣瀬 農; 田野井 慶太朗; 中西 友子
    • Organizer
      第52回アイソトープ・放射線研究発表会
    • Place of Presentation
      東京大学
    • Year and Date
      2015-07-09
    • Related Report
      2015 Research-status Report
  • [Remarks] 東京大学 放射線植物生理学研究室 研究テーマ

    • URL

      http://park.itc.u-tokyo.ac.jp/radio-plantphys/themes.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2018-03-22  

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