Project/Area Number |
15K18764
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Agricultural environmental engineering/Agricultural information engineering
|
Research Institution | University of Tsukuba (2017) Kobe University (2016) The University of Tokyo (2015) |
Principal Investigator |
KANNO Satomi 筑波大学, 生命環境系, 特別研究員(RPD) (20586010)
|
Research Collaborator |
LEONHARDT Nathalie CEA Cadarache, BIAM, Researcher
VERY Anne-Alienor INRA, Montpellier, Researcher
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | カリウム輸送体 / セシウム / AtHAK5 / ファイトレメディエーション / フィトレメディエーション / イメージング / イオン輸送 |
Outline of Final Research Achievements |
This research project studied with the view to obtain basic knowledge about molecular mechanisms of cesium (Cs) uptake by planta. Among the potassium (K) transporter family of Arabidopsis thaliana, AtHAK5, encoding high-affinity transporter, is a key gene for root K uptake under low external K conditions. We studied AtHAK5 gene expression in plant modified K distribution by using skor mutant that has less translocation rate of K from root to shoot. Interestingly, AtHAK5 expression decreased in skor mutant. This result suggests that AtHAK5 expression is regulated by internal K status and not only by external K concentration. In addition, since AtHAK5 is also a main contributor for Cs uptake, we evaluated Cs accumulation properties of skor. Our results show that skor is able to accumulate 50 % less Cs compared to the wild type due to down regulation of AtHAK5. Altogether, we demonstrate that AtHAK5 is regulated by external and internal K status and is essential for both K and Cs uptake.
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