Sutudy of the effect of external and internal ion status on the uptake of cesium.

• Project/Area Number: 15K18764
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: University of Tsukuba (2017); Kobe University (2016); The University of Tokyo (2015)
• Principal Investigator: KANNO Satomi 筑波大学, 生命環境系, 特別研究員(RPD) (20586010)
• Research Collaborator: LEONHARDT Nathalie CEA Cadarache, BIAM, Researcher ; VERY Anne-Alienor INRA, Montpellier, Researcher
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: カリウム輸送体 / セシウム / AtHAK5 / ファイトレメディエーション / フィトレメディエーション / イメージング / イオン輸送

Outline of Final Research Achievements

This research project studied with the view to obtain basic knowledge about molecular mechanisms of cesium (Cs) uptake by planta. Among the potassium (K) transporter family of Arabidopsis thaliana, AtHAK5, encoding high-affinity transporter, is a key gene for root K uptake under low external K conditions. We studied AtHAK5 gene expression in plant modified K distribution by using skor mutant that has less translocation rate of K from root to shoot. Interestingly, AtHAK5 expression decreased in skor mutant. This result suggests that AtHAK5 expression is regulated by internal K status and not only by external K concentration. In addition, since AtHAK5 is also a main contributor for Cs uptake, we evaluated Cs accumulation properties of skor. Our results show that skor is able to accumulate 50 % less Cs compared to the wild type due to down regulation of AtHAK5. Altogether, we demonstrate that AtHAK5 is regulated by external and internal K status and is essential for both K and Cs uptake.

Research Products

• [Int'l Joint Research] CEA / Cadarache/INRA / Montpellier(フランス)
• [Int'l Joint Research] CEA / Cadarache/INRA / Montpellier(フランス)
• [Int'l Joint Research] CEA/Cadarache/INRA/Montpellier(France)
• [Journal Article] A novel role for the root cap in phosphate uptake and homeostasis. (2016)
  • Author(s): Kanno S, Arrighi J-F, Chiarenza S, Bayle V, Berthome R, Peret B, Javot H, Delannoy E, Marin E, Nakanishi TM, Thibaud M-C and Nussaume L.
  • Journal Title: eLife Volume: 5 Pages: 1-16
  • DOI: 10.7554/elife.14577
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Performance and Limitations of Phosphate Quantification: Guidelines for Plant Biologists. (2016)
  • Author(s): Kanno S, Cuyas L, Javot H, Bligny R, Gout E, Dartevelle T, Hanchi M, Nakanishi TM, Thibaud MC, Nussaume L.
  • Journal Title: Plant Cell Physiol. Volume: -
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana. (2016)
  • Author(s): Bonnot C, Pinson B, Clément M, Bernillon S, Chiarenza S, Kanno S, Kobayashi N, Delannoy E, NakanishiI TM, Nussaume L, Desnos T.
  • Journal Title: New Phytol. Volume: 209 Issue: 1 Pages: 161-76
  • DOI: 10.1111/nph.13591
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Contribution of SKOR to Cesium absorption and translocation. (2018)
  • Author(s): Satomi KANNO, Ludovic MARTIN, Tatsuya NOBORI, Laurent NUSSAUME, Arain VAVASSEUR, Nathalie Leonhardt
  • Organizer: Seminaire du programme Toxicologie nucleaire
  • Int'l Joint Research
• [Presentation] Internal and external K+ status affect the expression of the high affinity K+ transporter AtHAK5. (2017)
  • Author(s): Satomi KANNO, Ludovic MARTIN, Tatsuya NOBORI, Laurent NUSSAUME, Arain VAVASSEUR, Nathalie Leonhardt
  • Organizer: XIV France-Japan Workshop on Plant Science 2017 "The developing plant in its environment"
  • Int'l Joint Research
• [Presentation] シロイヌナズナのリン酸濃度応答遺伝子の探索 (2017)
  • Author(s): 鈴木太郎, 大西美輪, 菅野里美, 豊倉浩一, 郷達明, 手塚あゆみ, 永野惇, 石川亮, 石崎公庸, 深城英弘, 三村徹郎
  • Organizer: 近畿植物学会
• [Presentation] A shortened annual cycle system; a tool for laboratory studies of seasonal phenomena in trees (2017)
  • Author(s): Yuko Kurita, Satomi Kanno, Ryohei Sugita, Atsushi Hirose, Miwa Ohnishi, Ayumi Tezuka, Ayumi Deguchi, Keiko Kosuge, Kimitsune Ishizaki, Hidehiro Fukaki, Keitaro Tanoi, Tomoko M. Nakanishi, Kei’ichi Baba, Tetsuro Mimura, Atsushi J. Nagano
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research
• [Presentation] シロイヌナズナのリン応答におけるBT遺伝子の機能の解析 (2016)
  • Author(s): 菅野里美、岡田和哉、前川修吾、大西美輪、石田哲也、Laurent NUSSAUME、中西友子、深城英弘、栁澤修一、三村徹郎
  • Organizer: 日本土壌肥料学会年会2016年度佐賀大会
  • Place of Presentation: 佐賀大学
  • Year and Date: 2016-09-20
• [Presentation] リン投与によるBT2遺伝子の発現誘導 (2015)
  • Author(s): 菅野里美、岡田和哉、大西美輪、Laurent NUSSAUME、三村徹郎、柳澤修一
  • Organizer: 日本土壌肥料学会2015年度京都大会
  • Place of Presentation: 京都大学
  • Year and Date: 2015-09-09
• [Presentation] リン投与によるBT2遺伝子の発現誘導 (2015)
  • Author(s): 菅野里美、岡田和哉、大西美輪、Laurent NUSSAUME、三村徹郎、柳澤修一
  • Organizer: 第一回植物栄養学会
  • Place of Presentation: 東京大学
  • Year and Date: 2015-09-04
• [Presentation] Imaging analysis of phosphate absorption and distribution of Plants (2015)
  • Author(s): Satomi KANNO, Marie-Christine THIBAUD, Atsushi HIROSE, Keitaro TANOI, Laurent NUSSAUME, Tomoko M. NAKANISHI
  • Organizer: The 26th International Conference on Arabidopsis Research
  • Place of Presentation: Parais des congres (Paris)
  • Year and Date: 2015-07-05
  • Int'l Joint Research