Improvement of oocyte competence by enhancing EGFR signaling in cattle
| Project/Area Number |
15K18768
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Sugimura Satoshi 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (00728454)
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| Research Collaborator |
Gilchrist Robert B ニューサウスウェルズ大学, 特任准教授
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | EGFR / cAMP / 卵子分泌因子 / 卵丘細胞卵子複合体 / ウシ / BMP15 / GDF9 / SMAD2/3 / SMAD1/5/8 / 卵丘細胞 / 卵子の発生能 / 発生能 / 卵成熟 / EGF受容体 |
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| Outline of Final Research Achievements |
Enhancement of EGF receptor (EGFR) signaling is milestone for acquisition of oocyte developmental competence. However, there are limited information in the mechanism. In preset study, we observed that FSH administration prior to ovum pick up improved oocyte competence. RNA-seq showed that cumulus cells receiving FSH-priming were enhanced EGFR signaling. To mimic the FSH administration in vitro, cumulus-oocyte compelexs (COCs) were tread with cAMP modulator, also known as the second messengers of FSH, before in vitro maturation (pre-IVM). The pre-IVM improved oocyte comptence and ehnaced EGFR signaling. Hence, cAMP may enhance EGFR signaling in bovine COCs.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Cumulin, an Oocyte-secreted Heterodimer of the Transforming Growth Factor-β Family, Is a Potent Activator of Granulosa Cells and Improves Oocyte Quality.2015
Author(s)
Mottershead DG, Sugimura S, Al-Musawi SL, Li JJ, Richani D, White MA, Martin GA, Trotta AP, Ritter LJ, Shi J, Mueller TD, Harrison CA, Gilchrist RB.
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Journal Title
Journal of Biological Chemistry
Volume: 290
Issue: 39
Pages: 24007-24020
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Presentation] Transcriptomic signature of the follicular somatic compartment surrounding an oocyte with high developmental competence2017
Author(s)
Sugimura S, Kobayashi N, Okae H, Yamanouchi T, Matsuda H, Kojima T, Yajima A, Hashiyada Y, Kaneda M, Sato K, Imai K, Tanemura K, Arima T, Gilchrist RB
Organizer
World Congress of Reproductive Biology
Related Report
Int'l Joint Research
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[Presentation] EGF受容体シグナリングの促進は低発生能卵子の品質を改善する2015
Author(s)
杉村智史，J Lesley Ritter, D Ryan Rose, G Jeremy Thompson, Johan Smith, G David Mottershead, B Robert Gilchrist
Organizer
第56回日本卵子学会
Place of Presentation
栃木県総合文化センター
Year and Date
2015-05-30
Related Report
