| Project/Area Number |
15K18768
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Sugimura Satoshi 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (00728454)
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| Research Collaborator |
Gilchrist Robert B ニューサウスウェルズ大学, 特任准教授
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | EGFR / cAMP / 卵子分泌因子 / 卵丘細胞卵子複合体 / ウシ / BMP15 / GDF9 / SMAD2/3 / SMAD1/5/8 / 卵丘細胞 / 卵子の発生能 / 発生能 / 卵成熟 / EGF受容体 |
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| Outline of Final Research Achievements |
Enhancement of EGF receptor (EGFR) signaling is milestone for acquisition of oocyte developmental competence. However, there are limited information in the mechanism. In preset study, we observed that FSH administration prior to ovum pick up improved oocyte competence. RNA-seq showed that cumulus cells receiving FSH-priming were enhanced EGFR signaling. To mimic the FSH administration in vitro, cumulus-oocyte compelexs (COCs) were tread with cAMP modulator, also known as the second messengers of FSH, before in vitro maturation (pre-IVM). The pre-IVM improved oocyte comptence and ehnaced EGFR signaling. Hence, cAMP may enhance EGFR signaling in bovine COCs.
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