Pathological study on the formation of ectopic granule cells in the hippocampus of rat model of epilepsy

• Project/Area Number: 15K18781
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Yamaguchi University
• Principal Investigator: SAKURAI Masashi 山口大学, 共同獣医学部, 助教 (00747967)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経病理 / 脳 / てんかん / 海馬 / 神経 / 神経新生 / アポトーシス

Outline of Final Research Achievements

Ectopic granule cells, which is observed in the hilus of the hippocampus, is considered to relate the epileptogenesis or progression of epileptic signs due to the formation of aberrant neural network. However, the mechanisms of ectopic granule cell formation are not well known. In this study, we immunohistochemically examined the brain of pilocarpine-treated rat of epilepsy model. From our results, it is suggested that microglial activation and survivin expression in the immature neurons are involved in the mechanisms of ectopic granule cell formation.

Research Products

• [Journal Article] Enhanced neurogenesis and possible synaptic reorganization in the piriform cortex of adult rat following kainic acid-induced status epilepticus (2018)
  • Author(s): Sakurai M., Suzuki H., Tomita N., Sunden Y., Shimada A., Miyata H., Morita T.
  • Journal Title: Neuropathology Volume: 38 Issue: 2 Pages: 135-143
  • DOI: 10.1111/neup.12445
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of a novel model for anti-cancer drug resistance in three-dimensional primary culture of tumor microenvironment. (2016)
  • Author(s): Usui T, Sakurai M, Enjoji S, Kawasaki H, Umata K, Ohama T, Fujiwara N, Yabe R, Tsuji S, Yamawaki H, Hazama S, Takenouchi H, Nakajima M, Tsunedomi R, Suzuki N, Nagano H and Sato K.
  • Journal Title: Stem Cells International. Volume: - Issue: 1 Pages: 7053872-7053872
  • DOI: 10.1155/2016/7053872
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Programmed Cell Death Ligand 1 Expression in Canine Cancers (2016)
  • Author(s): KAZUHA SHOSU, MASASHI SAKURAI, KUMI INOUE, TAKAYUKI NAKAGAWA, HIROKI SAKAI, MASAHIRO MORIMOTO, MASARU OKUDA, SHUNSUKE NOGUCHI, TAKUYA MIZUNO
  • Journal Title: in vivo Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Neurolymphomatosis in a cat (2016)
  • Author(s): Masashi Sakurai, Kazushi Azuma, Arata Nagai, Toru Fujioka, Yuji Sunden, Akinori Shimada, Takehito Morita
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 6 Pages: 1063-1066
  • DOI: 10.1292/jvms.15-0553
  • NAID: 130005159651
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cutaneous histiocytic sarcoma with E-cadherin expression in a Pembroke Welsh Corgi dog. (2015)
  • Author(s): Ayano Hirako, Akihiko Sugiyama, Masashi Sakurai, Kiyokazu Ozaki, Hiroki Sakai, Takashi Takeuchi, Takehito Morita, Peter F. Moore
  • Journal Title: Journal of Veterinary Diagnostic Investigation Volume: 27 Issue: 5 Pages: 589-595
  • DOI: 10.1177/1040638715604185
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Pathogenesis of Pancreatitis in Chickens after Experimental Infection with 9a5b Newcastle Disease Virus Mutant Isolate. (2015)
  • Author(s): Amanallah El-Bahrawy, Anis Zaid, Yuji Sunden, Masashi Sakurai,Hiroshi Ito, Toshihiro Ito, Takehito Morita
  • Journal Title: Journal of Comparative Pathology Volume: 153 Issue: 4 Pages: 315-323
  • DOI: 10.1016/j.jcpa.2015.08.002
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] リチウム-ピロカルピンてんかんモデルラットの異常な海馬の神経新生における細胞死と細胞生存についての検討 (2017)
  • Author(s): 櫻井 優、坂井 祐介、森本 將弘
  • Organizer: 第58回日本神経病理学会総会学術研究会
• [Presentation] Th2型免疫応答誘導モデルであるNippostrongylus brasiliensis感染マウスにおける成体脳の神経新生に関する病理学的検討 (2016)
  • Author(s): 倉地 淳也、櫻井 優、坂井 祐介、岡本 圭介、森本 將弘
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学湘南キャンパス（神奈川県藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] Th2型免疫応答モデルマウス確立への試み (2016)
  • Author(s): 岡本 圭介、坂井 祐介、櫻井 優、倉地 淳也、森本 將弘
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学湘南キャンパス（神奈川県藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] カイニン酸投与てんかんモデルラットの梨状葉皮質における神経新生に関する病理学的研究 (2015)
  • Author(s): 櫻井 優
  • Organizer: 第56回日本神経病理学会総会学術研究会
  • Place of Presentation: 九州大学医学部百年講堂（福岡県福岡市）
  • Year and Date: 2015-06-03