Development of metastasis factor for canine and feline cancer by plasma free amino acids

• Project/Area Number: 15K18793
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Tottori University
• Principal Investigator: Azuma Kazuo 鳥取大学, 農学部, 准教授 (50721841)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: アミノ酸 / 腫瘍 / 犬 / 脳腫瘍 / 臨床獣医学 / がん / 代謝

Outline of Final Research Achievements

In this study, the efficiency of plasma free amino acids levels (PFAA) as a metastasis factor for canine and feline cancer is investigated. We developed the simple and quick analysis method for canine PFAA levels. We observed food intakes affects PFAA levels in dogs. In the canine brain tumor patients, some PFAA levels were altered according to the brain tumor. These results indicate that we might tumor metastasis by measuring PFAA levels in companion animals.

Research Products

• [Journal Article] Analysis of plasma free amino acid profiles in canine brain tumors. (2017)
  • Author(s): S. Utsugi#, K. Azuma#, T. Osaki, Y. Murahata, T. Tsuka, N. Ito, T. Imagawa, Y. Okamoto
  • Journal Title: Biomedical Reports Volume: 6 Issue: 2 Pages: 195-200
  • DOI: 10.3892/br.2016.825
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Application of Pre-Column Labeling Liquid Chromatography for Canine Plasma-Free Amino Acid Analysis. (2016)
  • Author(s): K. Azuma*, Y. Hirao, Y. Hayakawa, Y. Murahata, T. Osaki, T. Tsuka, T. Imagawa, Y. Okamoto, N. Ito*
  • Journal Title: Metabolites Volume: 6 Issue: 1 Pages: 3-3
  • DOI: 10.3390/metabo6010003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 犬の各種疾患が血漿中アミノ酸濃度に及ぼす影響 (2017)
  • Author(s): 東 和生、窪田大樹、大智宏佑、村端悠介、大崎智弘、柄 武志、今川智敬、岡本芳晴、伊藤典彦
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Pre-column labeling liquid chromatography for canine plasma free amino acid analysis (2017)
  • Author(s): Kazuo Azuma, Yoshiharu Okamoto, Norihiko Ito
  • Organizer: 11th International Medical Chemistry Symposium (AIMECS)
  • Int'l Joint Research
• [Presentation] 犬における血漿遊離アミノ酸濃度測定の意義 (2017)
  • Author(s): 東 和生、山下真路、大崎智弘、村端悠介、柄 武志、今川智敬、岡本芳晴、伊藤典彦
  • Organizer: 平成29年度獣医学術中国地区学会
• [Presentation] 血中アミノ酸濃度変化が教えてくれる動物の疾患 (2016)
  • Author(s): 東 和生，村端悠介，大﨑智弘，伊藤典彦，柄 武志，山岸淑恵，勝呂 栞，今川智敬，岡本芳晴
  • Organizer: 第58回比較統合医療学会
  • Place of Presentation: 武蔵野市(東京都)
  • Year and Date: 2016-12-11
• [Presentation] 獣医療におけるLC-MS-MSを用いたゾ二サミドの治療薬物モニタリング (2016)
  • Author(s): 東 和生，駒田瞬太郎，国広彩耶，荒木佳織，大﨑智弘，今川智敬，柄 武志，村端悠介，岡本芳晴，伊藤典彦
  • Organizer: 第41回日本医用マススペクトル学会年会
  • Place of Presentation: 名古屋(愛知県)
  • Year and Date: 2016-09-15
• [Presentation] 犬の血液中アミノ酸濃度に影響を及ぼす各種背景因子の検討 (2016)
  • Author(s): 東 和生，平尾美子，松村有里子，岩澤篤郎，村端悠介，大﨑智弘，柄 武志，今川
  • Organizer: 日本アミノ酸学会10周年記念大会
  • Place of Presentation: 文京区(東京都)
  • Year and Date: 2016-09-11
• [Presentation] 犬の血漿中アミノ酸濃度に影響を及ぼす因子の検討 (2016)
  • Author(s): 東 和生，平尾美子，松村有里子，岩澤篤郎，村端悠介，大﨑智弘，柄 武志，今川
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 藤沢(神奈川県)
  • Year and Date: 2016-09-06
• [Presentation] 自動プレカラム誘導体法を用いた犬脳疾患における血中アミノ酸濃度測定 (2015)
  • Author(s): 東 和生，宇津木真一，衛藤展功，村端悠介，大﨑智弘，今川智敬，柄 武志，岡本芳晴，伊藤典彦
  • Organizer: 第91回日本獣医麻酔外科学会
  • Place of Presentation: SORA札幌コンベンションセンター
  • Year and Date: 2015-12-19
• [Presentation] 自動プレカラム誘導体法を用いた高速犬血漿アミノ酸分析法の確立 (2015)
  • Author(s): 東 和生，平尾美子，松村有里子，岩澤篤朗，村端悠介，大﨑智弘，今川智敬，柄 武志，岡本芳晴，伊藤典彦
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学獣医学部
  • Year and Date: 2015-09-07