Study on improvement of cryotolerance in bovine in vitro fertilized embryos by the regulation of aquaporin expression
| Project/Area Number |
15K18805
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Hokkaido Research Organization |
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Principal Investigator |
FUJII TAKASHI 地方独立行政法人北海道立総合研究機構, 農業研究本部畜産試験場, 研究職員 (60609105)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ウシ / 体外受精胚 / 凍結保存 / アクアポリン / 耐凍性 / 体外受精 / 初期胚 |
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| Outline of Final Research Achievements |
In the present study, we clarified the expression status of AQP3 and AQP7 mRNA and protein during bovine preimplantation development. Furthermore, we demonstrated that hypertonic treatment induces the up-regulation of AQP3 and AQP7 expression in bovine in vitro fertilized (IVF) embryos, and improves the viability of bovine IVF embryos after freeze-thawing and vitrification-warming. These findings suggest that AQP3 and AQP7 may be involved in the cryotolerance of bovine embryos, and artificial regulation of AQP3 and AQP7 expression may contribute the improvement of cryotolerance of bovine IVF embryos.
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Report
(4 results)
Research Products
(2 results)
