| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
In the present study, we clarified the expression status of AQP3 and AQP7 mRNA and protein during bovine preimplantation development. Furthermore, we demonstrated that hypertonic treatment induces the up-regulation of AQP3 and AQP7 expression in bovine in vitro fertilized (IVF) embryos, and improves the viability of bovine IVF embryos after freeze-thawing and vitrification-warming. These findings suggest that AQP3 and AQP7 may be involved in the cryotolerance of bovine embryos, and artificial regulation of AQP3 and AQP7 expression may contribute the improvement of cryotolerance of bovine IVF embryos.
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