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Ecological function of wood insect associated microbes

Research Project

Project/Area Number 15K18812
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental agriculture(including landscape science)
Research InstitutionHokkaido University

Principal Investigator

Takasuka Taichi  北海道大学, 農学研究院, 助教 (70748409)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords木質成分分解性微生物 / プロテオミクス / insect-microbe symbiont / Wood wasp / cellulolytic microbes / proteomics / biochemistry
Outline of Final Research Achievements

In this study, we attempted to identify the woodwasp symbiont microbes from Hokkaido forest. We captured two wood wasp species, Sirex nitobei and Xiphydria cannelus. One bacteria and one fungus were isolated from a mygcangia of X. canelus, and were identified as Rahnella spp., and Daldinia decipiens species by 16s rRNA and ITS sequencing, respectively. Furthermore, we determined the hemicellulose-degrading activity in the supernatant when D. decipiens was cultured in the liquid medium containing polysaccharides such as cellulose, hemicellulose, wood biomass as sole carbon source, and glucose was used as a control culture. By utilizing proteomics, we were able to determined multiple glycoside hydrolase family enzymes, which are predicted to break down various polysaccharides present in woody biomass.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (3 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] 森林生態系におけるキバチ共生細菌による木質分解2016

    • Author(s)
      高須賀太一、堀千明、James Ellinger、飛松裕基
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 54 Issue: 3 Pages: 156-158

    • DOI

      10.1271/kagakutoseibutsu.54.156

    • NAID

      130005241176

    • ISSN
      0453-073X, 1883-6852
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Characterization of biomass degradation by fungal and bacterial enzymes from wood devastating insect symbionts2016

    • Author(s)
      HORI Chiaki, SONG Ruopu, TOBIMATSU Yuki, MINKOFF Benjamin, TAKASUKA Taichi
    • Organizer
      日本生化学会年会
    • Place of Presentation
      仙台国際センター(宮城県仙台)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of lignin degradation by fungal and bacterial enzymes from wood devastating insect symbionts2016

    • Author(s)
      堀千明、飛松裕基、Benjamin B. Minkoff、 種田修造、 原秀穂、高須賀太一
    • Organizer
      京都大学生存圏ミニシンポジウム
    • Place of Presentation
      京都大学生存圏研究所(京都府・宇治市)
    • Year and Date
      2016-03-04
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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