Growth phase transition regulated by nutrient availability in plants

• Project/Area Number: 15K18819
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Hokkaido University
• Principal Investigator: Sato Takeo 北海道大学, 理学研究院, 助教 (50609724)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞機能 / 環境適応 / 栄養素代謝 / プロテオミクス / 成長相転換

Outline of Final Research Achievements

Nutrient availability, in particularly the balance of carbon (C) and nitrogen (N) is one of the most important environmental cue regulating plant metabolism and growth phase transition. However detailed mechanisms regulating C/N balance response are not well understood. Transcriptome and phosphoproteome analyses identified several candidates of signaling factors mediating C/N-dependent flowering in Arabidopsis plants. Further genetic and physiological analyses revealed molecular basis of crosstalk between C/N-nutrient and flowering signals.

Research Products

• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] University of Washington(米国)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] University of Washington/University of Minnesota(米国)
• [Journal Article] Direct transcriptional activation of BT genes by NLP transcription factors is a key component of the nitrate response in Arabidopsis (2017)
  • Author(s): Sato, T., Maekawa, S., Konishi, M., Yoshioka, N., Sasaki, Y., Maeda, H., Ishida, T., Kato, Y., Yamaguchi, J. and Yanagisawa, S.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 483 Issue: 1 Pages: 380-386
  • DOI: 10.1016/j.bbrc.2016.12.135
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Arabidopsis CBL-interacting protein kinases regulate carbon/nitrogen-nutrient response by phosphorylating ubiquitin ligase ATL31. (2017)
  • Author(s): Yasuda S, Aoyama S, Hasegawa Y, Sato T, Yamaguchi J
  • Journal Title: Mol. Plant Volume: 10 Issue: 4 Pages: 605-618
  • DOI: 10.1016/j.molp.2017.01.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ubiquitin related enzymes and plant-specific ubiquitin ligase ATL family in tomato plants (2017)
  • Author(s): Li X, Hasegawa Y, Lu Y and Sato T
  • Journal Title: Plant Biotechnology Volume: 印刷中
  • NAID: 130006789285
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of ubiquitin ligase SlATL31 and proteomic analysis of 14-3-3 targets in tomato fruit tissue (Solanum lycopersicum L.) (2016)
  • Author(s): Lu Y., Yasuda S., Li W., Fukao Y., Tohge T., Fernie A.R., Matsukura C., Ezura H., Sato T. and Yamaguchi J.
  • Journal Title: Journal of Proteomics Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Integration of C/N-nutrient and multiple environmental signals into the ABA signaling cascade (2015)
  • Author(s): Lu Y., Yamaguchi J. and Sato T.
  • Journal Title: Plant Signaling & Behavior Volume: 12 Issue: 12 Pages: e1048940-e1048940
  • DOI: 10.1080/15592324.2015.1048940
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ABI1 regulates carbon/nitrogen-nutrient signal transduction independent of ABA biosynthesis and canonical ABA signalling pathways in Arabidopsis (2015)
  • Author(s): Lu, Y. et al.
  • Journal Title: Journal of Experimental Botany Volume: 66 Issue: 9 Pages: 2763-2771
  • DOI: 10.1093/jxb/erv086
  • Peer Reviewed / Open Access
• [Presentation] Ubiquitin signal involved in membrane trafficking for plant environmental stress responses (2017)
  • Author(s): Takeo Sato, Yoko Hasegawa, Shigetaka Yasuda and Junji Yamaguchi
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学(鹿児島県・鹿児島市)
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] 翻訳後修飾による新規植物基幹代謝制御機構の解明 (2016)
  • Author(s): 佐藤長緒
  • Organizer: 日本植物細胞分子生物学会（上田）大会・シンポジウム
  • Place of Presentation: 信州大学繊維学部（長野県・上田市）
  • Year and Date: 2016-09-01
  • Invited
• [Presentation] Arabidopsis CBL-Interacting Protein Kinases Regulate Carbon/Nitrogen-Nutrient Response by Phosphorylating Ubiquitin Ligase ATL31 (2016)
  • Author(s): Takeo Sato, Shigetaka Yasuda and Junji Yamaguchi
  • Organizer: EMBO conference: The nitrogen nutrition of plants (Nitrogen 2016)
  • Place of Presentation: Montpellier (France)
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Phosphoproteome analysis of carbon/nitrogen nutrient signal transduction in Arabidopsis plant (2016)
  • Author(s): Sato T., Aoyama S., Nomura Y., Nakagami H., Ito S., Abe M., Imaizumi T. and Yamaguchi J.
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学（岩手県・盛岡市）
  • Year and Date: 2016-03-18
• [Presentation] Receptor-like kinase LMK1 integrating C/N-nutrient and pathogen signal transduction (2015)
  • Author(s): Sato T.
  • Organizer: 第4回エンドメンブレンミーティング
  • Place of Presentation: 北海道大学農学部（北海道・札幌市）
  • Year and Date: 2015-09-28
• [Presentation] Membrane-localized ubiquitin ligase ATL31 associated with TGN-SNAREs regulates plant response to nutrient availability (2015)
  • Author(s): Sato T., Huarancca Reyes T., Uemura T., Fukao Y., Nakano A. and Yamaguchi J.
  • Organizer: EMBO conference Signalling in Plant Development
  • Place of Presentation: Bruno (Czech Republic)
  • Year and Date: 2015-09-20
  • Int'l Joint Research
• [Presentation] 植物の炭素/窒素栄養バランス応答を制御する膜局在型ユビキチンリガーゼATL31の機能解析 (2015)
  • Author(s): 佐藤長緒，Thais HUARANCCA REYES，植村知博，深尾陽一朗，山口淳二
  • Organizer: 日本土壌肥料学会2015年度京都大会
  • Place of Presentation: 京都大学（京都府・京都市）
  • Year and Date: 2015-09-09
• [Presentation] 栄養による植物免疫の制御 (2015)
  • Author(s): 佐藤長緒
  • Organizer: 第１回植物の栄養研究会
  • Place of Presentation: 東京大学弥生キャンパス（東京都・文京区）
  • Year and Date: 2015-09-04
  • Invited
• [Presentation] ユビキチンリガーゼATL31と小胞輸送制御因子の相互作用による植物C/N栄養応答制御 (2015)
  • Author(s): 佐藤長緒，Thais HUARANCCA REYES，植村知博，深尾陽一朗，山口淳二
  • Organizer: 日本植物細胞分子生物学会（東京）大会・シンポジウム
  • Place of Presentation: 東京大学弥生キャンパス（東京都・文京区）
  • Year and Date: 2015-08-10
• [Remarks] 所属講座穂ホームページ
  • URL: http://www.sci.hokudai.ac.jp/CSF2-web/
• [Remarks] 所属講座ホームページ
  • URL: http://www.sci.hokudai.ac.jp/CSF2-web/