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Elucidation of the molecular mechanism of heavy metal tolerance and accumulation in copper moss by transcriptome analysis and genome editing technology

Research Project

Project/Area Number 15K18824
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Nomura Toshihisa  国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (20722771)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsホンモンジゴケ / 重金属耐性 / コケ植物 / ゲノム編集 / CRISPR/Cas9システム
Outline of Final Research Achievements

In order to develop technologies for heavy metal phytoremediation, it is important to elucidate the heavy metal tolerance and accumulation mechanism of the hyperaccumulator plants. In this study, we focused on copper mosses Scopelophila cataractae which has copper tolerance and accumulation ability. By using genomic editing technology and transcriptome analysis, we aimed to elucidate the molecular mechanisms underlying copper tolerance and accumulation in S. cataractae. As a result, we found that the mechanism of copper export from intracellular to extracellular via copper transporter ScHMA5 is necessary for copper tolerance ability in S. cataractae.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Invited: 1 results)

  • [Journal Article] Targeted Mutagenesis Using RNA-guided Endonucleases in Mosses2017

    • Author(s)
      Nomura T, Sakakibara H
    • Journal Title

      Bio-protocol

      Volume: 7(12) Issue: 12

    • DOI

      10.21769/bioprotoc.2359

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Efficient and heritable targeted mutagenesis in mosses using the CRISPR/Cas9 system2016

    • Author(s)
      T. Nomura, T. Sakurai, Yuriko Osakabe, Keishi Osakabe and H. Sakakibara
    • Journal Title

      Plant & Cell Physiology

      Volume: 57 Issue: 12 Pages: 2600-2610

    • DOI

      10.1093/pcp/pcw173

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] コケ植物におけるニッチ戦略のための細胞分化-銅苔における無性芽の分化-2016

    • Author(s)
      野村俊尚, 馳澤盛一郎, 榊原均
    • Journal Title

      BSJ-reviw (植物科学の最前線)

      Volume: 7D Pages: 153-160

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ゲノム編集技術で紐解くホンモンジゴケの銅耐性機構 (シンポジウム: 植物機能の解明を目指すゲノム編集技術)2017

    • Author(s)
      野村俊尚、櫻井哲也、刑部祐里子、刑部敬史、馳澤盛一郎、榊原均
    • Organizer
      日本植物生理学会第58回年会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県・鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 銅輸送体遺伝子は、ホンモンジゴケの銅耐性に関与する2017

    • Author(s)
      野村俊尚、櫻井哲也、井藤賀操、馳澤盛一郎、榊原均
    • Organizer
      日本蘚苔類学会第46回群馬大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Copper tolerance mechanism via copper transporter in Scopelophila cataractae2017

    • Author(s)
      野村俊尚、井藤賀操、檜垣匠、櫻井哲也、馳澤盛一郎、榊原均
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRISPR/Cas9システムによる蘚類の高効率ゲノム編集2016

    • Author(s)
      野村俊尚、榊原均
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] CRISPR/Cas9システムによる蘚類(ホンモンジゴケおよびヒメツリガネゴケ)の高効率ゲノム編集2016

    • Author(s)
      櫻井哲也、刑部祐里子、刑部敬史、榊原均
    • Organizer
      日本ゲノム編集学会第1回大会
    • Place of Presentation
      広島国際会議場(広島県・広島市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 蘚類へのゲノム編集技術CRISPR/Cas9システムの導入2016

    • Author(s)
      野村俊尚、榊原均
    • Organizer
      日本蘚苔類学会第45 回大会
    • Place of Presentation
      屋久島町安房総合センター (鹿児島県・屋久島町)
    • Year and Date
      2016-08-29
    • Related Report
      2016 Research-status Report
  • [Presentation] Contribution of Copper-transporting P-type ATPase to copper tolerance in coppermoss Scopelophila cataractae2016

    • Author(s)
      Nomura T, Itouga M, Kato Y, Hasezawa S, Sakakibara H
    • Organizer
      日本植物生理学会第57回年会
    • Place of Presentation
      岩手大学上田キャンパス (岩手県・盛岡市)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Research-status Report
  • [Presentation] コケ植物にみられるニッチ戦略のための細胞分化 (シンポジウム: 細胞機能の変容と循環を視る~可逆性と不可逆性から探る 細胞分化の本質~)2015

    • Author(s)
      野村俊尚、若崎眞由美、上原由紀子、吉田拓広、小嶋美紀子、豊岡公徳、持田恵一、櫻井哲也、馳澤盛一郎、榊原均
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      新潟コンベンションセンター (新潟県・新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Research-status Report
  • [Presentation] ホンモンジゴケの無性芽に関する基礎的な解析2015

    • Author(s)
      野村俊尚、馳澤盛一郎 、榊原均
    • Organizer
      日本蘚苔類学会第44回大会
    • Place of Presentation
      生涯学習館花の郷・茂来館 (長野県・佐久穂町)
    • Year and Date
      2015-08-04
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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