Development of a method for distribution analysis of sphingolipids in tissue by mass spectrometry

• Project/Area Number: 15K18842
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: Saigusa Daisuke 東北大学, 東北メディカル・メガバンク機構, 講師 (90545237)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: スフィンゴ脂質 / レーザーマイクロダイセクション / LC-MS/MS / ヒートスタビライザー / IMS / 質量分析計 / S1P

Outline of Final Research Achievements

In this study, we developed a method for demonstrating tissue distribution of sphingolipids by means of LC-MS/MS and IMS.
We first optimized a time or temperature of heat stabilizer for sphingolipids analysis in tissue. Then, the distribution of sphingolipids in a part of white and red pulp of spleen was demonstrated by LC-MS/MS and IMS, and the concentration of sphingolipids was significantly higher in red pulp than white pulp (P < 0.05). In addition, we demonstrated that the concentration of sphingolipids related with the expression of SPHK or SPL by LC-MS/MS.

Research Products

• [Journal Article] Establishment of protocols for global metabolomics by LC-MS for biomarker discovery (2016)
  • Author(s): Saigusa D, Okamura Y, Motoike IN, Katoh Y, Kurosawa Y, Saijyo R, Koshiba S, Yasuda J, Motohashi H, Sugawara J, Tanabe O, Kinoshita K, Yamamoto M
  • Journal Title: PLoS One Volume: 11 Issue: 8 Pages: e0160555-e0160555
  • DOI: 10.1371/journal.pone.0160555
  • Peer Reviewed / Open Access
• [Journal Article] Sphingosine kinase-1, S1P transporter spinster homolog 2 and S1P2 mRNA expressions are increased in liver with advanced fibrosis in human (2016)
  • Author(s): Sato M, Ikeda H, Uranbileg B, Kurano M, Saigusa D, Aoki J, Maki H, Kudo H, Hasegawa K, Kokudo N, Yatomi Y.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 32119-32119
  • DOI: 10.1038/srep32119
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Increased mRNA Levels of Sphingosine Kinases and S1P Lyase and Reduced Levels of S1P Were Observed in Hepatocellular Carcinoma in Association with Poorer Differentiation and Earlier Recurrence (2016)
  • Author(s): Uranbileg B, Ikeda H, Kurano M, Enooku K, Sato M, Saigusa D, Aoki J, Ishizawa T, Hasegawa K, Kokudo N, Yatomi Y.
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0149462-e0149462
  • DOI: 10.1371/journal.pone.0149462
  • Peer Reviewed / Open Access
• [Journal Article] A novel and sensitive assay for heme oxygenase activity (2015)
  • Author(s): Iwamori S, Sato E, Saigusa D, Yoshinari K, Ito S, Sato H, and Takahashi N
  • Journal Title: Am J Physiol Renal Physiol Volume: 309 Issue: 7 Pages: F667-F671
  • DOI: 10.1152/ajprenal.00210.2015
  • Peer Reviewed
• [Journal Article] Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases (2015)
  • Author(s): Suzuki T, Yamaguchi H, Kikusato M, Matsuhashi T, Matsuo A, Sato T, Oba Y, Watanabe S, Minaki D, Saigusa D, Shimbo H, Mori N, Mishima E, Shima H, Akiyama Y, Takeuchi Y, Yuri A, Kikuchi K, Toyohara T, Suzuki C, Kohzuki M, Anzai J, Mano N, Kure S, Yanagisawa T, Tomioka Y, Toyomizu M, Ito S, Osaka H, Hayashi K, Abe T
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 236 Issue: 3 Pages: 225-232
  • DOI: 10.1620/tjem.236.225
  • NAID: 130005083526
  • ISSN: 0040-8727, 1349-3329
  • URL: https://pure.teikyo.jp/en/publications/f34848a7-3f52-481e-850d-fae28c8f317e
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Omics Research in a Large-scale Cohort by MS (2017)
  • Author(s): Daisuke Saigusa
  • Organizer: BIT's 5th Annual Conference of AnalytiX 2017
  • Place of Presentation: Hilton Fukuoka Sea Hawk（福岡県・福岡市）
  • Year and Date: 2017-03-22
  • Int'l Joint Research / Invited
• [Presentation] 大規模メタボロームデータの多層オミクス解析への応用 (2016)
  • Author(s): 三枝大輔、元池育子、小柴生造
  • Organizer: 第10回メタボロームシンポジウム
  • Place of Presentation: 鶴岡メタボロームキャンパス（山形県・鶴岡市）
  • Year and Date: 2016-10-19
• [Presentation] Transomics analysis in a large-scale cohort study (2016)
  • Author(s): 三枝大輔
  • Organizer: 日本プロテオーム学会2016年大会
  • Place of Presentation: 北里大学薬学部白金キャンパス（東京都・港区）
  • Year and Date: 2016-07-28
  • Invited
• [Presentation] 質量分析計を用いる生体内スフィンゴ脂質の安定性評価と臨床領域への応用 (2016)
  • Author(s): 三枝大輔、王嬌、可野邦行、B.Uranbileg、蔵野信、池田均、矢冨裕、青木淳賢
  • Organizer: 第64回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク（大阪府・吹田市）
  • Year and Date: 2016-05-18
• [Presentation] 大規模コホートオミクス解析による疾患予防マーカー探索 (2015)
  • Author(s): 三枝大輔
  • Organizer: 第40回日本医用マススペクトル学会
  • Place of Presentation: アクトシティ浜松、静岡県浜松市
  • Year and Date: 2015-09-17
• [Presentation] 臨床メタボローム解析による疾患予防マーカー探索 (2015)
  • Author(s): 三枝大輔
  • Organizer: 第36回グアニジノ化合物研究会
  • Place of Presentation: 東北大学大学院薬学研究科、宮城県仙台市
  • Year and Date: 2015-08-08
• [Presentation] Development of Protocol for Global Metabolomics in Clinical and Cohort Study by LC-MS (2015)
  • Author(s): Daisuke Saigusa, Junichi Sugawara, Mitsuyo Matsumoto, Yasutake Katoh, Ryu Yamashita, Yasuhiro Kurosawa, Jun Yasuda, Masayuki Yamamoto, Osamu Tanabe
  • Organizer: Metabolomics 2015
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] 臨床領域における質量分析計を基盤としたメタボローム解析の発展 (2015)
  • Author(s): 三枝大輔
  • Organizer: 第63回質量分析総合討論会
  • Place of Presentation: つくば国際会議場、茨城県つくば市
  • Year and Date: 2015-06-17
  • Invited
• [Presentation] Global Metabolomic Analysis by UPLC-TOF-MS for Identification of Predictive and Prognostic Biomarkers for Obstetric Complications (2015)
  • Author(s): Daisuke Saigusa, Junichi Sugawara, Mitsuyo Matsumoto, Yasutake Katoh, Ryu Yamashita, Yasuhiro Kurosawa, Jun Yasuda, Osamu Tanabe, Masayuki Yamamoto
  • Organizer: 8th International DIP Symposium on Diabetes, Hypertension, Metabolic Syndrome & Pregnancy
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2015-04-15
  • Int'l Joint Research
• [Book] 「腎臓のサイエンス」腎臓のバイオマーカーと創薬戦略、実験医学 (2016)
  • Author(s): 鈴木健弘、三島英換、三枝大輔
  • Total Pages: 1355
  • Publisher: 羊土社