Development of a method for distribution analysis of sphingolipids in tissue by mass spectrometry
Project/Area Number |
15K18842
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Physical pharmacy
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Research Institution | Tohoku University |
Principal Investigator |
Saigusa Daisuke 東北大学, 東北メディカル・メガバンク機構, 講師 (90545237)
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | スフィンゴ脂質 / レーザーマイクロダイセクション / LC-MS/MS / ヒートスタビライザー / IMS / 質量分析計 / S1P |
Outline of Final Research Achievements |
In this study, we developed a method for demonstrating tissue distribution of sphingolipids by means of LC-MS/MS and IMS. We first optimized a time or temperature of heat stabilizer for sphingolipids analysis in tissue. Then, the distribution of sphingolipids in a part of white and red pulp of spleen was demonstrated by LC-MS/MS and IMS, and the concentration of sphingolipids was significantly higher in red pulp than white pulp (P < 0.05). In addition, we demonstrated that the concentration of sphingolipids related with the expression of SPHK or SPL by LC-MS/MS.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Establishment of Protocols for Global Metabolomics by LC-MS for Biomarker Discovery.2016
Author(s)
Saigusa D, Okamura Y, Motoike IN, Katoh Y, Kurosawa Y, Saijyo R, Koshiba S, Yasuda J, Motohashi H, Sugawara J, Tanabe O, Kinoshita K, Yamamoto M
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Journal Title
PLoS One
Volume: 11
Issue: 8
Pages: e0160555-e0160555
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sphingosine kinase-1, S1P transporter spinster homolog 2 and S1P2 mRNA expressions are increased in liver with advanced fibrosis in human.2016
Author(s)
Sato M, Ikeda H, Uranbileg B, Kurano M, Saigusa D, Aoki J, Maki H, Kudo H, Hasegawa K, Kokudo N, Yatomi Y.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 32119-32119
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Increased mRNA Levels of Sphingosine Kinases and S1P Lyase and Reduced Levels of S1P Were Observed in Hepatocellular Carcinoma in Association with Poorer Differentiation and Earlier Recurrence.2016
Author(s)
Uranbileg B, Ikeda H, Kurano M, Enooku K, Sato M, Saigusa D, Aoki J, Ishizawa T, Hasegawa K, Kokudo N, Yatomi Y.
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Journal Title
PLoS One
Volume: 11
Issue: 2
Pages: e0149462-e0149462
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Mitochonic Acid 5 (MA-5), a Derivative of the Plant Hormone Indole-3-Acetic Acid, Improves Survival of Fibroblasts from Patients with Mitochondrial Diseases2015
Author(s)
Suzuki T, Yamaguchi H, Kikusato M, Matsuhashi T, Matsuo A, Sato T, Oba Y, Watanabe S, Minaki D, Saigusa D, Shimbo H, Mori N, Mishima E, Shima H, Akiyama Y, Takeuchi Y, Yuri A, Kikuchi K, Toyohara T, Suzuki C, Kohzuki M, Anzai J, Mano N, Kure S, Yanagisawa T, Tomioka Y, Toyomizu M, Ito S, Osaka H, Hayashi K, Abe T
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Journal Title
The Tohoku Journal of Experimental Medicine
Volume: 236
Issue: 3
Pages: 225-232
DOI
NAID
ISSN
0040-8727, 1349-3329
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Development of Protocol for Global Metabolomics in Clinical and Cohort Study by LC-MS2015
Author(s)
Daisuke Saigusa, Junichi Sugawara, Mitsuyo Matsumoto, Yasutake Katoh, Ryu Yamashita, Yasuhiro Kurosawa, Jun Yasuda, Masayuki Yamamoto, Osamu Tanabe
Organizer
Metabolomics 2015
Place of Presentation
San Diego, CA, USA
Year and Date
2015-06-28
Related Report
Int'l Joint Research
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[Presentation] Global Metabolomic Analysis by UPLC-TOF-MS for Identification of Predictive and Prognostic Biomarkers for Obstetric Complications2015
Author(s)
Daisuke Saigusa, Junichi Sugawara, Mitsuyo Matsumoto, Yasutake Katoh, Ryu Yamashita, Yasuhiro Kurosawa, Jun Yasuda, Osamu Tanabe, Masayuki Yamamoto
Organizer
8th International DIP Symposium on Diabetes, Hypertension, Metabolic Syndrome & Pregnancy
Place of Presentation
Berlin, Germany
Year and Date
2015-04-15
Related Report
Int'l Joint Research
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