| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We hypothesized that exposure of low concentration of doxorubicin (DXR) would cause cell cycle arrest in G2/M phase which is supposed to be a suitable phase for transgene expression and therefore enhance transgene expression. Then we evaluated whether transgene expression by liposomal pDNA was enhanced by DXR and G2/M cell cycle arrest was concurrent with the enhancement. As a result, indeed gene expression was enhanced by DXR treatment, but G2/M arrest was caused in a small fraction of cell population. This result implied that other factors would contribute to the enhancement of gene expression by DXR treatment. Then we analyzed the confocal images of the cells incorporating liposomal pDNA. The data showed the significant increase of nuclear sectional size and promotion of nuclear entry of liposomal pDNA.
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