| Project/Area Number |
15K18846
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Keio University |
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Principal Investigator |
Hiruta Yuki 慶應義塾大学, 薬学部(芝共立), 助教 (60710944)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 温度 / pH / 機能性高分子 / ミセル / DDS / 抗がん剤 / ナノ材料 / pH応答性ポリマー / 温度応答性ポリマー / ブロックコポリマー / ポリマーミセル / ドラッグデリバリー / ドキソルビシン / ドラッグデリバリーシステム / がん / ナノ |
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| Outline of Final Research Achievements |
We report a polymeric micelle drug delivery system, which enables selective intracellular uptake with external thermal stimulation, and effective release of a drug at internal acidic endosomal pH. We developed dual temperature- and pH-responsive polymeric micelle encapsulated anti-cancer agent, doxorubicin (DOX). The resultant micelle exhibited a temperature-dependent phase transition at a temperature slightly higher than body temperature, and intracellular uptake of encapsulated DOX was accelerated above phase transition temperature. The cytotoxicity of doxorubicin (DOX)-loaded dual temperature- and pH-responsive micelles against human cervical cancer HeLa cells was significantly greater at 42 °C than at 37 °C, This proof-of-concept synergistic two-step delivery system with enhanced intracellular uptake upon external thermal stimulation and rapid release of DOX at internal acidic endosomal pH was effective against tumor cells in vitro.
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