Development of electron micrscopy technique for observation of interaction between polymeric nano carriers and bacteria
| Project/Area Number |
15K18849
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | National Institute of Advanced Industrial Science and Technology (2018)
Aichi Gakuin University (2015-2017) |
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Principal Investigator |
Takahashi Chisato 国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (50574448)
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| Research Collaborator |
Muto Shunsuke
Yagi Shinya
Yamamoto Hiromitsu
Nagano Keiji
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | バイオフィルム感染症 / ドラッグデリバリーシステム / 高分子キャリア / 電子顕微鏡 / イオン液体 / カソードルミネッセンス / 高分子製剤 / イメージング / XAFS / ドラッグデリバリー / 高分子粒子製剤 / 金属ナノ粒子 / バイオフィルム / ナノ粒子 / レーザーラマン顕微鏡 / 製剤設計 |
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| Outline of Final Research Achievements |
To optimize the preparation technique of drug delivery system particles for biofilm infections, we developed sample pretreatment technique using an ionic liquid and scanning transmission electron microscopy-cathodoluminescence. Especially, CL imaging and CL analysis of polymeric particles in the biofilm was successfully performed by attaching the fluorescent materials. These developed techniques could be effective to design of polymeric particles for treating biofilm infection diseases.
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| Academic Significance and Societal Importance of the Research Achievements |
上記の「研究成果の概要」に記載した新規の評価法を組み込むことで、有効なバイオフィルム感染症治療薬が開発できれば、治療の短期化による医療費の抑制や多剤耐性菌の発現リスク低減などが期待される。また、本研究成果は歯周病が引き起こしうる白内障や糖尿病、心筋梗塞などの疾病予防にも繋がるため社会的波及効果は大きい。また、学術的観点から、従来の試料作製法では形態維持が難しいとされる微小器官の構造解析が可能となり、微生物のみでなく動植物の生体機構解明及び未知なる機能探索にも大きく寄与するものと考えられる。
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Report
(5 results)
Research Products
(43 results)
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[Journal Article] Imaging of intracellular behavior of polymeric nanoparticles in Staphylococcus epidermidis biofilms by slit-scanning confocal Raman microscopy and scanning electron microscopy with energy-dispersive X-ray spectroscopy.2017
Author(s)
Takahashi, C., Ueno, K., Aoyama, J., Adachi, M.., Yamamoto, H.
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Journal Title
Mater. Sci. Eng. C,
Volume: 印刷中
Pages: 1066-1074
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Visualization of silver-decorated poly (DL-lactide-co-glycolide) nanoparticles and their efficacy against Staphylococcus epidermidis.2017
Author(s)
Takahashi, C., Matsubara, N., Akachi, Y., Ogawa, N., Golap, K., Asaka, T., Tanemura, M., Kawashima, Y., Yamamoto, H.
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Journal Title
Mater. Sci. Eng. C,
Volume: 72
Pages: 143-149
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Morphological study of efficacy of clarithromycin-loaded nanocarriers for treatment of biofilm infection disease.2016
Author(s)
Takahashi, C., Akachi, Y., Ogawa, N., Moriguchi, K., Asaka, T., Tanemura, M., Kawashima, Y., Yamamoto, H.
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Journal Title
Med. Mol. Morphol.
Volume: -
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Antibacterial activities of polymeric poly (DL-lactide-co-glycolide) nanoparticles and Soluplus® micelles against Staphylococcus epidermidis biofilm and their characterization.2015
Author(s)
Takahashi, C., Saito, S., Suda, A., Ogawa, N., Kawashima, Y., Yamamoto, H.
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Journal Title
RSC adv.
Volume: 5
Issue: 88
Pages: 71709-71717
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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