The role of protein arginine methyltransferase (PRMT) in brain tumor

• Project/Area Number: 15K18858
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: Hiyoshi Hiromi 名古屋市立大学, 大学院医学研究科, 助教 (10406530)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 脳腫瘍 / アルギニンメチル化酵素 / 細胞周期

Outline of Final Research Achievements

It is known that the protein arginine methyltransferase (PRMT) 8 is a brain-specific member of the PRMT family. But, PRMT8 was not detected in glioma cell lines. We tried to over-express of PRMT8 in glioma cells. Over-expression of PRMT8 suppressed cell proliferation in glioma cell lines. There was no change in arginine methylation of protein, although it is the main action of PRMT family. Therefore, we investigated the expression of protein involved cancer cell proliferation. The level of Rb protein, key factor of cell cycle, was decreased in glioma cells over-expressed with PRMT8. These results suggest that PRMT8 inhibits the cell proliferation of brain tumor by novel mechanism.

Research Products

• [Journal Article] Cullin-3 and its adaptor protein ANKFY1 determine the surface level of integrin beta1 in endothelial cells. (2017)
  • Author(s): Maekawa M, Tanigawa K, Sakaue T, Hiyoshi H, Kubota E, Joh T, Watanabe Y, Taguchi T, Higashiyama S
  • Journal Title: Biolology Open Volume: 6 Pages: 1707-1719
  • DOI: 10.1242/bio.029579
  • Peer Reviewed / Open Access
• [Journal Article] Neddylated Cullin 3 is required for vascular endothelial-cadherin-mediated endothelial barrier function. (2017)
  • Author(s): Sakaue T, Fujisaki A, Nakayama H, Maekawa M, Hiyoshi H, Kubota E, Joh T, Izutani H, Higashiyama S.
  • Journal Title: Cancer Sci. Volume: 108 Issue: 2 Pages: 208-215
  • DOI: 10.1111/cas.13133
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prognostic significance of immunohistochemical expression of the ubiquitin ligase carboxyl terminus of Hsc70-interacting protein (CHIP) in postmenopausal women with invasive breast cancer (2016)
  • Author(s): Kurozumi S, Yamaguchi Y, Hayashi S, Hiyoshi H, Suda T, Matsumoto H, Takei H, Horiguchi J, Oyama T, Takeyoshi I, Kurosumi M
  • Journal Title: Cancer Med Volume: 5(8) Issue: 8 Pages: 1873-1882
  • DOI: 10.1002/cam4.780
  • Peer Reviewed / Open Access
• [Journal Article] CHIP acts as a capacitor of phenotypic heterogeneity in breast cancer cells (2014)
  • Author(s): Tsuchiya M, Nakajima Y, Waku T, Hiyoshi H, Morishita T, Furumai R, Hayashi Y, Kishimoto H, Kimura K, Yanagisawa J.
  • Journal Title: Oncogene Volume: なし Issue: 35 Pages: 1-8
  • DOI: 10.1038/onc.2014.387
  • Peer Reviewed