Transcriptional regulatory mechanism of the RNA polymerase II initiation complex by the chromatin remodeling complex
| Project/Area Number |
15K18861
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biological pharmacy
|
|---|
| Research Institution | University of Toyama |
|---|
Principal Investigator |
Tanaka Aki 富山大学, 大学院医学薬学研究部(薬学), 助教 (50432109)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 遺伝子発現 / 転写 / 基本転写因子 / 遺伝子発現制御 / 生化学 / 転写開始 / 基本転写因子TFIIE / RNAポリメラーゼII clamp領域 / TFIIE / RNAポリメラーゼ clamp |
|---|
| Outline of Final Research Achievements |
We performed Mass spectrometry to identify TFIIEα binding proteins. It was indicated that TFIIEα interacted with the SWI/SNF complex which is one of the chromatin remodeling complexes. The SWI/SNF complex can be divided into BAF and PBAF. Our immunoprecipitation study demonstrated that TFIIE interacted with the PBAF mainly and TFIIH. TFIIE recruits TFIIH to complete the PIC formation and regulates enzymatic activities of TFIIH. There is an interesting possibility that TFIIE-TFIIH act on the promoter coordinately with the PBAF complex and regulates transcription and the chromatin structure together.
|
Report
(4 results)
Research Products
(21 results)
-
[Journal Article] Mediator cyclin-dependent kinases upregulate transcription of inflammatory genes in cooperation with NF-κB and C/EBPβ on stimulation of Toll-like receptor 9.2017
Author(s)
Yamamoto, S., Hagihara, T., Horiuchi, Y., Okui, A., Wani, S., Yoshida, T., Inoue, T., Tanaka, A., Ito, T., Hirose, Y., and Ohkuma, Y.
-
Journal Title
GENES TO CELLS
Volume: 22
Issue: 3
Pages: 265-276
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Association of the Winged Helix Motif of the TFIIEα Subunit of TFIIE with Either the TFIIEβ Subunit or TFIIB Distinguishes Its Functions in Transcription.2015
Author(s)
Tanaka, A., Akimoto, Y., Kobayashi, S., Hisatake, K., Hanaoka, F., and Ohkuma, Y.
-
Journal Title
Genes Cells
Volume: 20
Issue: 3
Pages: 203-216
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Human mediator MED17 subunit plays essential roles in gene regulation by associating with the transcription and DNA repair machineries.2014
Author(s)
Kikuchi, Y., Umemura, H., Nishitani, S., Iida, S., Fukasawa, R., Hayashi, H., Hirose, Y., Tanaka, A., Sugasawa, K. and Ohkuma, Y.
-
Journal Title
Genes to Cells
Volume: 20
Issue: 3
Pages: 191-202
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
