| Project/Area Number |
15K18862
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
Sawada Hitoshi 名古屋大学, 大学院理学研究科附属臨海実験所, 所長・教授
Shirae Maki (Kurabayashi Maki) 名古屋大学, 大学院理学研究科附属臨海実験所, 技術職員
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 受精 / 精子 / ホヤ / タンパク質 / 生化学 / プロテオミクス / 卵外被 / ライシン / 自家不和合性 |
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| Outline of Final Research Achievements |
Sperm should overcome barriers, including the long distance to the egg and the hard membrane protecting the egg (egg coat), to achieve fertilization. We attempted to reveal how sperm penetrate through the prohibiting egg coat through the ascidian Ciona robusta (kata yurei boya), a handy model animal for fertilization assays. First we inventoried the sperm surface proteins, that could participate in interaction with the egg coat, to acquire the basic knowledge for further analyses. Among those proteins, metalloproteases, a kind of enzymes, were abundant. We found that metalloproteases were requisite for fertilization, especially for degradation of the egg coat.
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