Role of Rab family proteins in regulation of antigen presentation in dendritic cells

• Project/Area Number: 15K18864
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Okayama University
• Principal Investigator: Furuta Kazuyuki 岡山大学, 医歯薬学総合研究科, 准教授 (50644936)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 抗原提示 / 樹状細胞 / MHC-II / Rab / エンドサイトーシス / Rab11 / リサイクリング

Outline of Final Research Achievements

MHC-II expressed on antigen presenting cells such as dendritic cells presents antigenic peptides to CD4 T cells and initiates acquired immunity. The cell-surface expression of MHC-II is tightly regulated, however, the regulation mechanism remains to be clarified. In this study we obtained the following results: 1. MHC-II closslinking induced intracellular Ca2+ influx was required for MHC-II endocytosis. 2. LPS induced Rab11 expression in dendritic cells.

Research Products

• [Journal Article] 1-Fluoro-2,4-dinitrobenzene and its derivatives act as secretagogues on rodent mast cells. (2017)
  • Author(s): Manabe, Y., Yoshimura, M., Sakamaki, K., Inoue, A., Kakinoki, A., Hokari, S., Sakanaka, M., Aoki, J., Miyachi, H., Furuta, K., and Tanaka, S.
  • Journal Title: Eur. J. Immunol. Volume: 47 Issue: 1 Pages: 60-67
  • DOI: 10.1002/eji.201646536
  • Peer Reviewed
• [Journal Article] Vesicular Polyamine Transporter Mediates Vesicular Storage and Release of Polyamine from Mast Cells (2017)
  • Author(s): Tomoya Takeuchi, Yuika Harada, Satomi Moriyama, Kazuyuki Furuta, Satoshi Tanaka, Takaaki Miyaji, Hiroshi Omote, Yoshinori Moriyama, and Miki Hiasa
  • Journal Title: The Journal of Biological Chemistry Volume: 292(9) Issue: 9 Pages: 3909-3918
  • DOI: 10.1074/jbc.m116.756197
  • Peer Reviewed / Open Access
• [Journal Article] Down-modulation of antigen-induced activation of murine cultured mast cells sensitized with a highly cytokinergic IgE clone. (2016)
  • Author(s): Sakanaka M, Kurimune Y, Yamada K, Hyodo N, Natsuhara M, Ichikawa A, Furuta K, Tanaka S.
  • Journal Title: Immunology letter Volume: 174 Pages: 1-8
  • DOI: 10.1016/j.imlet.2016.04.003
  • Peer Reviewed
• [Journal Article] Cell surface-anchored fluorescent probe capable of real-time imaging of single mast cell degranulation based on histamine-induced coordination displacement. (2016)
  • Author(s): Oshikawa, Y., Furuta, K., Tanaka, S., and Ojida, A.
  • Journal Title: Anal. Chem. Volume: 88 Issue: 3 Pages: 1526-1529
  • DOI: 10.1021/acs.analchem.5b04758
  • Peer Reviewed
• [Presentation] 主要組織適合抗原クラスII (MHC－II)の誘導するシグナルを介したMHC-IIのエンドサイトーシス機構 (2017)
  • Author(s): 古田和幸 平木勇次 政木健人 田中智之
  • Organizer: 日本薬学会 第137年会
  • Place of Presentation: 仙台市
  • Year and Date: 2017-03-25
• [Presentation] MHC-IIシグナルが誘導するMHC-IIのエンドサイトーシス促進機構の解析 (2016)
  • Author(s): 古田和幸、平木勇次、田中智之
  • Organizer: 第８９回日本生化学会大会
  • Place of Presentation: 仙台市
  • Year and Date: 2016-09-25
• [Presentation] 主要組織適合抗原クラスII (MHC-II)のエンドサイトーシスを促進するシグナル伝達機構の解析 (2016)
  • Author(s): 古田和幸、平木勇次、田中智之
  • Organizer: 日本薬学会第１３６年会
  • Place of Presentation: 横浜市
  • Year and Date: 2016-03-26
• [Presentation] 主要組織適合抗原クラスIIのリサイクリングおよび発現を制御するRabの解析 (2015)
  • Author(s): 古田和幸、黒田真弘、田中智之
  • Organizer: 第38回日本分子生物学会年会第88回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2015-12-01
• [Presentation] 主要組織適合抗原クラスIIのリサイクリングを制御するRabの解析 (2015)
  • Author(s): 古田和幸、黒田真弘、田中智之
  • Organizer: 第67回日本細胞生物学会大会
  • Place of Presentation: 東京都
  • Year and Date: 2015-06-30
• [Remarks] 岡山大学薬学部田中研究室
  • URL: http://www.pharm.okayama-u.ac.jp/lab/meneki/