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Carboxyl-terminal tail-mediated homodimerizations of sphingomyelin synthases are responsible for efficient export from the endoplasmic reticulum

Research Project

Project/Area Number 15K18868
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionTeikyo University

Principal Investigator

Hayashi Yasuhiro  帝京大学, 薬学部, 助教 (70582857)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords脂質 / ホモダイマー
Outline of Final Research Achievements

Sphingomyelin synthase (SMS) is the key enzyme for cross-talk between bioactive sphingolipids and glycerolipids. Here, we demonstrate that both SMS1 and SMS2 form homodimers. Homodimer formation was significantly decreased by C-terminal truncations, SMS1-ΔC22 and SMS2-ΔC30, indicating that the C-terminal tails of the SMSs are primarily responsible for homodimer formation. Interestingly, homodimer formation occurred in the endoplasmic reticulum (ER) membrane before trafficking to the Golgi apparatus. Reduced homodimerization caused by C-terminal truncations of SMSs significantly reduced ER-to-Golgi transport. Our findings suggest that the C-terminal tails of SMSs are involved in homodimer formation, which is required for efficient transport from the ER.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Carboxyl-terminal tail-mediated homodimerizations of sphingomyelin synthases are responsible for efficient export from the endoplasmic reticulum2017

    • Author(s)
      Hayashi Y, Nemoto-Sasaki Y, Matsumoto N, Tanikawa T, Oka S, Tanaka Y, Arai S, Wada I, Sugiura T, Yamashita A.
    • Journal Title

      J Biol Chem.

      Volume: 292 Issue: 3 Pages: 1122

    • DOI

      10.1074/jbc.m116.746602

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Klotho-Related Protein KLrP: Structure and Functions2016

    • Author(s)
      Yasuhiro Hayashi and Makoto Ito
    • Journal Title

      Vitam Horm.

      Volume: 101 Pages: 1-16

    • DOI

      10.1016/bs.vh.2016.02.011

    • ISBN
      9780128048191
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Insights into the mechanism of inhibition of CXCR4: identification of Piperidinylethanamine analogs as anti-HIV-1 inhibitors2015

    • Author(s)
      Das D, Maeda K, Hayashi Y, Gavande N, Desai DV, Chang SB, Ghosh AK, Mitsuya H.
    • Journal Title

      Antimicrob Agents Chemother

      Volume: 59 Pages: 1895-1904

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] スフィンゴミエリン合成酵素のC末端を介したホモオリゴマー形成はゴルジ体への輸送に関与する2016

    • Author(s)
      林康広、佐々木洋子、松本直樹、荒井斉祐、和田郁夫、杉浦隆之、山下純
    • Organizer
      日本生化学会
    • Place of Presentation
      宮城県 仙台市 仙台国際センター
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Carboxyl-terminal tail-mediated homodimerizations of sphingomyelin synthases are responsible for efficient ER export2016

    • Author(s)
      Yasuhiro Hayashi, Yoko Nemoto-Sasaki, Naoki Matsumoto, Takashi Tanikawa, Saori Oka, Yusuke Tanaka, Seisuke Arai, Ikuo Wada, Takayuki Sugiura, and Atsushi Yamashita
    • Organizer
      Asia-Pacific Academic Consortium for Public Health Conference
    • Place of Presentation
      東京都 板橋区 帝京大学
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] スフィンゴミエリン合成酵素2はHIV-1の膜融合を促進する2016

    • Author(s)
      林 康広、佐々木洋子、松本直樹、光武 進、杉浦隆之、山下 純
    • Organizer
      Pharmaco-Hematology シンポジウム
    • Place of Presentation
      東京都 板橋区 帝京大学
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] スフィンゴミエリン合成酵素のオリゴマー形成にはC末端が重要である2016

    • Author(s)
      林康広、佐々木洋子、松本直樹、荒井斉祐、和田郁夫、杉浦隆之、山下純
    • Organizer
      スフィンゴテラピィ研究会
    • Place of Presentation
      石川県 加賀市 癒しのリゾート加賀の幸 ホテルアローレ
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] スフィンゴミエリン合成酵素のC末端を介したホモオリゴマー形成はゴルジ体への輸送に重要である2016

    • Author(s)
      林康広、佐々木洋子、松本直樹、荒井斉祐、和田郁夫、杉浦隆之、山下純
    • Organizer
      日本脂質生化学会
    • Place of Presentation
      秋田県 秋田市 にぎわい交流館AU
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] スフィンゴミエリン合成酵素オリゴマー形成領域の解析2015

    • Author(s)
      林康広、佐々木洋子、松本直樹、荒井斉祐、和田郁夫、杉浦隆之、山下純
    • Organizer
      第88回 日本生化学会
    • Place of Presentation
      兵庫県神戸市 神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Sphingomyelin synthase 2 is involved in HIV-1 envelope-mediated membrane fusion2015

    • Author(s)
      Yasuhiro Hayashi, Yoko Nemoto-Sasaki, Naoki Matsumoto, Takayuki Sugiura, and Atsushi Yamashita
    • Organizer
      第63回 日本ウィルス学会
    • Place of Presentation
      福岡県福岡市 福岡国際会議場
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] スフィンゴミエリン合成酵素2はHIV-1の膜融合を促進する2015

    • Author(s)
      林 康広、佐々木洋子、松本直樹、光武 進、杉浦隆之、山下 純
    • Organizer
      第57回 日本脂質生化学会
    • Place of Presentation
      東京都千代田区 一橋大学一橋講堂
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Remarks] 帝京大学ホームページ

    • URL

      https://www.e-campus.gr.jp/staffinfo/public/staff/detail/1463/16

    • Related Report
      2016 Annual Research Report
  • [Remarks] Research map

    • URL

      http://researchmap.jp/1549688131217/

    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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