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Cloning, expression, purification, and characterization of enzymes that cleave puerarin C-glucosidic bond.

Research Project

Project/Area Number 15K18891
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Natural medicines
Research InstitutionSuzuka University of Medical Science

Principal Investigator

Kenichi Nakamura  鈴鹿医療科学大学, 薬学部, 助手 (70512002)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsC-配糖体 / 腸内細菌 / プエラリン / 代謝酵素
Outline of Final Research Achievements

Puerarin is an isoflavone C-glucoside contained in the roots of Pueraria lobata Ohwi. We previously isolated a human intestinal bacterium, called strain PUE, which can metabolize puerarin to daidzein. In this study, cloning, expression, purification, and characterization of enzymes that cleave puerarin C-glucosidic bond from strain PUE were accomplished.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2017 2016 2015

All Presentation (4 results)

  • [Presentation] 腸内細菌由来C-配糖体代謝酵素の基質特異性の解析2017

    • Author(s)
      中村賢一、有竹萌音、鍋島渉、Shu ZHU、小松かつ子、服部征雄、岩島誠
    • Organizer
      日本薬学会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] プエラリン酸化物をダイゼインに代謝する腸内細菌由来酵素の機能解析2016

    • Author(s)
      中村賢一、大洲章裕、各務有里、川口宗一郎、Shu ZHU、小松かつ子、服部征雄、岩島誠
    • Organizer
      日本生薬学会
    • Place of Presentation
      富山国際会議場(富山県富山市)
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 腸内細菌由来C-配糖体マンギフェリン代謝酵素の精製と機能解析2016

    • Author(s)
      中村 賢一、黒川梨佐、大洲章裕、各務有里、川口宗一郎、Zhu Shu、小松かつ子、服部征雄、岩島誠
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 腸内細菌 strain PUE 由来プエラリンC-配糖体代謝酵素遺伝子群の同定2015

    • Author(s)
      中村賢一、河合礼薫、Zhu Shu、小松かつ子、服部征雄、岩島誠
    • Organizer
      第32回和漢医薬学会学術大会
    • Place of Presentation
      富山
    • Year and Date
      2015-08-22
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2018-03-22  

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