Cloning, expression, purification, and characterization of enzymes that cleave puerarin C-glucosidic bond.

• Project/Area Number: 15K18891
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: Kenichi Nakamura 鈴鹿医療科学大学, 薬学部, 助手 (70512002)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: C-配糖体 / 腸内細菌 / プエラリン / 代謝酵素

Outline of Final Research Achievements

Puerarin is an isoflavone C-glucoside contained in the roots of Pueraria lobata Ohwi. We previously isolated a human intestinal bacterium, called strain PUE, which can metabolize puerarin to daidzein. In this study, cloning, expression, purification, and characterization of enzymes that cleave puerarin C-glucosidic bond from strain PUE were accomplished.

Research Products

• [Presentation] 腸内細菌由来C-配糖体代謝酵素の基質特異性の解析 (2017)
  • Author(s): 中村賢一、有竹萌音、鍋島渉、Shu ZHU、小松かつ子、服部征雄、岩島誠
  • Organizer: 日本薬学会
  • Place of Presentation: 仙台国際センター（宮城県仙台市）
  • Year and Date: 2017-03-24
• [Presentation] プエラリン酸化物をダイゼインに代謝する腸内細菌由来酵素の機能解析 (2016)
  • Author(s): 中村賢一、大洲章裕、各務有里、川口宗一郎、Shu ZHU、小松かつ子、服部征雄、岩島誠
  • Organizer: 日本生薬学会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2016-09-24
• [Presentation] 腸内細菌由来C-配糖体マンギフェリン代謝酵素の精製と機能解析 (2016)
  • Author(s): 中村 賢一、黒川梨佐、大洲章裕、各務有里、川口宗一郎、Zhu Shu、小松かつ子、服部征雄、岩島誠
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] 腸内細菌 strain PUE 由来プエラリンC-配糖体代謝酵素遺伝子群の同定 (2015)
  • Author(s): 中村賢一、河合礼薫、Zhu Shu、小松かつ子、服部征雄、岩島誠
  • Organizer: 第32回和漢医薬学会学術大会
  • Place of Presentation: 富山
  • Year and Date: 2015-08-22