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Role of Mer transporters in the transport of toxic metals in Escherichia coli.

Research Project

Project/Area Number 15K18907
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Environmental and hygienic pharmacy
Research InstitutionKitasato University

Principal Investigator

SONE Yuka  北里大学, 薬学部, 助教 (60550035)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords水銀耐性遺伝子 / 有害金属輸送活性 / 複合汚染浄化 / 有害金属複合汚染 / ファイトレメディエーション
Outline of Final Research Achievements

The characteristics of bacteria take up mercury into cells via Mer superfamily, i.e., MerC, MerE, MerF, or MerT, have been applied in engineering of bioreactor used for mercurial bioremediation. Our objective was to clarify individual role of MerC, MerE, MerF, or MerT and potential in transport of toxic metals. The E. coli cells that carried control vector or mer recombinants were suspended in LB broth containing Cd(II), As(III), or Cr(VI) and incubated for various time, respectively. The cells that expressed MerC, MerE, MerF, or MerT accumulated significantly more Cd, or As than control. MerC recombinants accumulated significantly more Cr than control. Consequently, we demonstrated that MerC, MerE, MerF, and MerT are broad-spectrum toxic metals transporters. Our results suggested that all Mer transporters can be used for designing toxic metals bioremediation systems, moreover, MerC is the most efficient tool.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016 2015 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 3 results) Remarks (1 results)

  • [Journal Article] A Novel Role of MerC in Methylmercury Transport and Phytoremediation of Methylmercury Contamination2017

    • Author(s)
      Sone Y, Uraguchi S, Takanezawa Y, Nakamura R, Pan-Hou H, Kiyono M
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 40 Issue: 7 Pages: 1125-1128

    • DOI

      10.1248/bpb.b17-00213

    • NAID

      130006846433

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cysteine and histidine residues are involved in Escherichia coli Tn21 MerE methylmercury transport.2017

    • Author(s)
      Sone Y, Uraguchi S, Takanezawa Y, Nakamura R, Pan-Hou H, Kiyono M.
    • Journal Title

      FEBS Open Bio

      Volume: 7 Issue: 12 Pages: 1994-1999

    • DOI

      10.1002/2211-5463.12341

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 水銀トランスポーターMerCの金属選択性に関する研究2018

    • Author(s)
      曽根有香、金子莉子、浦口晋平、中村亮介、高根沢康一、清野正子
    • Organizer
      日本薬学会第138年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水銀トランスポーターMerC, MerE, MerF, MerTのヒ素およびクロム輸送に関する研究2017

    • Author(s)
      曽根有香、浦口晋平、中村亮介、高根沢康一、清野正子
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Role of Mer in the transport of toxic metals in Escherichia coli.2017

    • Author(s)
      Yuka Sone, Shimpei Uraguchi, Ryosuke Nakamura, Yasukazu Takanezawa, Masako Kiyono
    • Organizer
      FEMS2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水銀トランスポーターMerCを利用したメチル水銀高蓄積性植物の作出2016

    • Author(s)
      曽根有香、浦口晋平、中村亮介、高根沢康一、清野正子
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-26
    • Related Report
      2015 Research-status Report
  • [Presentation] Role of Mer in the transport of mercurials in Escherichia coli2015

    • Author(s)
      Y. Sone, R. Nakamura, Y. Takanezawa, S. Uraguchi and M. Kiyono
    • Organizer
      PACIFICHEM 2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Role of MerC, MerE, MerF, or MerT in the transport of mercurials in Escherichia coli2015

    • Author(s)
      Y. Sone, R. Nakamura, Y. Takanezawa, S. Uraguchi and M. Kiyono
    • Organizer
      ICOBTE 2015
    • Place of Presentation
      福岡
    • Year and Date
      2015-07-12
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Remarks] 北里大学薬学部公衆衛生学教室ホームページ

    • URL

      http://www.pharm.kitasato-u.ac.jp/kouei/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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