| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The characteristics of bacteria take up mercury into cells via Mer superfamily, i.e., MerC, MerE, MerF, or MerT, have been applied in engineering of bioreactor used for mercurial bioremediation. Our objective was to clarify individual role of MerC, MerE, MerF, or MerT and potential in transport of toxic metals. The E. coli cells that carried control vector or mer recombinants were suspended in LB broth containing Cd(II), As(III), or Cr(VI) and incubated for various time, respectively. The cells that expressed MerC, MerE, MerF, or MerT accumulated significantly more Cd, or As than control. MerC recombinants accumulated significantly more Cr than control. Consequently, we demonstrated that MerC, MerE, MerF, and MerT are broad-spectrum toxic metals transporters. Our results suggested that all Mer transporters can be used for designing toxic metals bioremediation systems, moreover, MerC is the most efficient tool.
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