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Morphological approach to understanding the intracellular degradation machinery in endocrine cells

Research Project

Project/Area Number 15K18951
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General anatomy (including histology/embryology)
Research InstitutionObihiro University of Agriculture and Veterinary Medicine (2017-2018)
Asahikawa Medical College (2015-2016)

Principal Investigator

Bochimoto Hiroki  帯広畜産大学, 畜産学部, 特任准教授 (60632841)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords電子顕微鏡 / 免疫組織化学 / 細胞内小器官 / 小胞体 / ミトコンドリア / オートファジー / 細胞・組織 / 粗面小胞体 / ゴルジ装置 / 内分泌細胞 / 実験内分泌学 / 下垂体前葉 / 性腺刺激ホルモン産生細胞 / 小胞体関連分解
Outline of Final Research Achievements

In this study, we analyzed the ultrastructural characteristics with functional molecular localization in rat pituitary gonadotropes under continuous administration of GnRH agonist. Upon continuous stimulation on the GnRH receptor, anomalous tubuloreticular ER patches appeared concomitant with functional suppression of gonadotropes. In the ER patches that appeared in the stimulated gonadotropes, ER molecular chaperons and ERAD-related molecules were accumulated. In parallel to appearance of anomalous ER patches, the accumulation of p62 and multilamellar components as reflection of activated autophagy processes appeared in cytoplasm of the stimulated gonadotropes. These findings indicated that ERAD may be related to autophagy in gonadotropes stimulated by a GnRH agonist.

Academic Significance and Societal Importance of the Research Achievements

本研究では細胞内微細構造と機能分子局在変化の正確な把握のために、光学顕微鏡、透過電顕および走査電顕技術を駆使・洗練する必要があり、本研究遂行によって確度の高い、精密な形態学的解析技術を確立することができた。また、本研究では性腺刺激ホルモン産生細胞における生理機能を形態学的側面と照合することで、同細胞における細胞内分解系に関わる新たな所見を発見でき、同細胞の生理機能の基盤解明に貢献した。これらの基盤的知見は今後、応用的に性ホルモンが関わる疾患の診断・治療に役立つ可能性があると考えられる。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (22 results)

All 2019 2018 2017 2016 2015 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 3 results) Presentation (10 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Ultrastructural changes in colonic epithelial cells in a rat model of inflammatory bowel disease2019

    • Author(s)
      Bochimoto Hiroki、Kondoh Daisuke、Nagata Ryuji、Ishihara Yo、Tomiyasu Jumpei、Han Kyu‐Ho、Shimada Kenichiro、Sasaki Motoki、Kitamura Nobuo、Fukushima Michihiro
    • Journal Title

      Microscopy Research and Technique

      Volume: in press Issue: 8 Pages: 1-6

    • DOI

      10.1002/jemt.23285

    • NAID

      120006875733

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kallikrein 6 secreted by oligodendrocytes regulates the progression of experimental autoimmune encephalomyelitis2018

    • Author(s)
      Bando Y, Hagiwara Y, Suzuki Y, Yoshida K, Aburakawa Y, Kimura T, Murakami C, Ono M, Tanaka T, Jiang Y-P, Mitrovi B, Bochimoto H, Yahara O, Yoshida S
    • Journal Title

      Glia

      Volume: 66 Issue: 2 Pages: 359-378

    • DOI

      10.1002/glia.23249

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Impaired processing of prohormones in secretogranin III null mice causes maladaptation to an inadequate diet and stress.2018

    • Author(s)
      Yoshinori Maeda, Saki Kudo, Ken Tsushima, Eri Sato, Chisato Kubota, Aika Kayamori, Hiroki Bochimoto, Daisuke Koga, Seiji Torii, Hiroshi Gomi, Tsuyoshi Watanabe, Masahiro Hosaka
    • Journal Title

      Endocrinology

      Volume: 159 Issue: 2 Pages: 1213-1227

    • DOI

      10.1210/en.2017-00636

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The ultrastructural characteristics of porcine hepatocytes donated after cardiac death and preserved with warm machine perfusion preservation2017

    • Author(s)
      Bochimoto H, Matsuno N, Ishihara Y, Shonaka T, Koga D, Hira Y, Nishikawa Y, Furukawa H, Watanabe T
    • Journal Title

      PLos One

      Volume: 12 Issue: 10 Pages: e0186352-e0186352

    • DOI

      10.1371/journal.pone.0186352

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] <b>Changes in the three-dimensional ultrastructure of membranous organelles in male rat pituitary gonadotropes after castration</b>2017

    • Author(s)
      Koga D, Bochimoto H, Kusumi S, Ushiki T, Watanabe T
    • Journal Title

      Biomedical Research

      Volume: 38 Issue: 1 Pages: 1-18

    • DOI

      10.2220/biomedres.38.1

    • NAID

      130005242939

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Zanthoxylum fruit extract from Japanese pepper promotes autophagic cell death in cancer cells.2016

    • Author(s)
      Reo Nozaki, Toru Kono, Hiroki Bochimoto, Tsuyoshi Watanabe, Kaori Oketani, Yuichi Sakamaki, Naoto Okubo, Koji Nakagawa, Hiroshi Takeda
    • Journal Title

      Oncotarget

      Volume: 7(43) Issue: 43 Pages: 70437-70446

    • DOI

      10.18632/oncotarget.11926

    • NAID

      120005906993

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Scanning Electron Microscopy Findings of Machine Perfused Liver Graft After Warm Ischemia Between Hypothermic and Rewarming Machine Perfusion in Pigs.2016

    • Author(s)
      Meng L, Matsuno N, Watanabe K, Furukori M, Obara H, Bochimoto H, Watanabe T, Fukukawa H
    • Journal Title

      Transplantation proceedings

      Volume: 48(7) Issue: 7 Pages: 2467-2470

    • DOI

      10.1016/j.transproceed.2016.03.059

    • NAID

      120006325063

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Backscattered electron image of osmium-impregnated/macerated tissues as a novel technique for identifying the cis-face of the Golgi apparatus by high-resolution scanning electron microscopy.2016

    • Author(s)
      Koga D, Bochimoto H, Watanabe T, Ushiki T
    • Journal Title

      J Microsc

      Volume: in press Issue: 1 Pages: 87-96

    • DOI

      10.1111/jmi.12379

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to their Molecular Components.2015

    • Author(s)
      Koga D, Kusumi S, Bochimoto H, Watanabe T, Ushiki T
    • Journal Title

      J Histochem Cytochem

      Volume: 63 Issue: 12 Pages: 968-979

    • DOI

      10.1369/0022155415609099

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 実験誘発性炎症性腸疾患モデルラットの大腸上皮細胞における微細構造の変化2018

    • Author(s)
      暮地本宙己、近藤大輔、韓圭鎬、佐々木基樹、福島道広、北村延夫
    • Organizer
      第64回東北・北海道連合支部学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 大学特性に適合した保健管理業務への取り組みの検討2018

    • Author(s)
      暮地本宙己、石原鮎美、守谷恭輔、栁川久
    • Organizer
      第56回全国大学保健管理研究集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 形態学的手法を用いた生命現象解明へのアプローチ~内分泌細胞における内膜系小器官の応答の解析を中心に~2017

    • Author(s)
      暮地本宙己
    • Organizer
      第35回北海道三大学獣医解剖学合同セミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 帯広畜産大学における保健管理業務への取り組みについて-学生・職員に対する日常的支援を中心として-2017

    • Author(s)
      暮地本宙己、伊藤鮎美、守谷恭輔、栁川久
    • Organizer
      第55回全国大学保健管理協会北海道地方部会研究集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 肝臓灌流保存における超微形態解析に基づく虚血再灌流障害の克服2017

    • Author(s)
      暮地本宙己、庄中達也、大谷将秀、合地美香子、石原洋、小原 弘道、古川 博之、松野直徒
    • Organizer
      第44回日本臓器保存生物医学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 持続的なGnRH刺激を受けたラットLH/FSH産生細胞における細胞内膜系の変化2017

    • Author(s)
      暮地本 宙己、渡部 剛
    • Organizer
      第22回グリアクラブ
    • Place of Presentation
      小樽
    • Related Report
      2016 Research-status Report
  • [Presentation] 前立腺上皮細胞におけるゴルジ装置の形態に性ホルモンが与える影響2016

    • Author(s)
      石原 洋、暮地本 宙己、甲賀 大輔、堀 淳一、渡部 剛
    • Organizer
      第121回日本解剖学会総会・全国学術集会
    • Place of Presentation
      福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 延髄腹外側部アドレナリン作動性ニューロンおよび結合腕傍核外側部への上行性投射系の神経化学的特性2016

    • Author(s)
      暮地本 宙己、今野 幸太郎、宮崎 太輔、山崎 美和子、渡部 剛、渡辺 雅彦
    • Organizer
      日本解剖学会第61回東北・北海道連合支部学術集会
    • Place of Presentation
      帯広
    • Related Report
      2016 Research-status Report
  • [Presentation] プロピルチオウラシル(PTU)投与後のラット下垂体前葉甲状腺刺激ホルモン(TSH)産生細胞における細胞内小器官の変化2015

    • Author(s)
      暮地本 宙己、甲賀 大輔、渡部 剛
    • Organizer
      平成27年度公益社団法人日本顕微鏡学会北海道支部学術講演会
    • Place of Presentation
      札幌
    • Year and Date
      2015-12-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 持続的なGnRH刺激を受けたラットLH/FSH産生細胞における細胞内膜系の変化と細胞内分解システムの関係2015

    • Author(s)
      暮地本 宙己、甲賀 大輔、板東 良雄、穂坂 正博、牛木 辰男、渡部 剛
    • Organizer
      日本解剖学会第61回東北・北海道連合支部学術集会
    • Place of Presentation
      盛岡
    • Year and Date
      2015-08-29
    • Related Report
      2015 Research-status Report
  • [Remarks]

    • URL

      https://researchmap.jp/bochimoto/

    • Related Report
      2018 Annual Research Report
  • [Remarks] researchmap

    • URL

      http://researchmap.jp/bochimoto/

    • Related Report
      2015 Research-status Report
  • [Remarks] 研究者詳細 - 暮地本 宙己 - 研究者総覧 - 旭川医科大学

    • URL

      http://hyouka04.asahikawa-med.ac.jp/html/100000383_ja.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

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