Mechanism for the maintenance of pseudostratified neuroepithelium

• Project/Area Number: 15K18953
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Nagoya University
• Principal Investigator: Shinoda Tomoyasu 名古屋大学, 医学系研究科, 助教 (80505652)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 神経発生 / 自己組織化 / 大脳発生 / 細胞運動 / 発生・分化

Outline of Final Research Achievements

Neuroepithelium is widely observed in developing central nervous systems including cerebral cortex. Neural progenitor cells in neuroepithelium show cell cycle-dependent somatic motion called interkinetic nuclear migration (INM) in apico-basal direction. The purpose of this study is to elucidate how neuroepithelium keep its pseudostratified structure over time in spite of every cell shows INM. We hypothesized stresses in neuroepithelium by in toto time-lapse observation of all nuclei in developing cerebral cortex. Mathematical simulations and in vivo experiments revealed that newborn G1-phase cells in subapical area utilize the restoring force of its surrounding cells for their basal-directed nucleokinesis. These results suggest that the physical properties arised from the neuroepitelial structure assist the displacement of somata/nuclei in subapical area to sustain the pseudostratification.

Research Products

• [Journal Article] Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics (2017)
  • Author(s): Mingyue Jin, Oz Pomp, Tomoyasu Shinoda, Shiori Toba, Takayuki Torisawa, Ken’ya Furuta, Kazuhiro Oiwa, Takuo Yasunaga, Daiju Kitagawa, Shigeru Matsumura, Takaki Miyata, Thong Teck Tan, Bruno Reversade & Shinji Hirotsune
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 39902-39902
  • DOI: 10.1038/srep39902
  • NAID: 120005997787
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Differences in the Mechanical Properties of the Developing Cerebral Cortical Proliferative Zone between Mice and Ferrets at both the Tissue and Single-Cell Levels. (2016)
  • Author(s): Nagasaka, A., Shinoda, T., Kawaue, T., Suzuki, M., Nagayama, K., Matsumoto, T., Ueno, N., Kawaguchi, A. and Miyata, T.
  • Journal Title: Front Cell Dev. Biol. Volume: 25 Pages: 139-139
  • DOI: 10.3389/fcell.2016.00139
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium. (2016)
  • Author(s): Sayaka Katsunuma, Hisao Honda, Tomoyasu Shinoda, Yukitaka Ishimoto, Takaki Miyata, Hiroshi Kiyonari, Takaya Abe, Ken-ichi Nibu, Yoshimi Takai, and Hideru Togashi.
  • Journal Title: The Journal of Cell Biology Volume: 212 Issue: 5 Pages: 561-75
  • DOI: 10.1083/jcb.201509020
  • NAID: 120005766825
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 大脳原基神経系前駆細胞で見出された新規の動的膜構造 (2017)
  • Author(s): 篠田 友靖、深澤 有吾、都島 悠佑、川上 巧、宮田 卓樹
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス(長崎県長崎市）
  • Year and Date: 2017-03-28
• [Presentation] Novel mode of action for interkinetic nuclear migration (2016)
  • Author(s): Tomoyasu Shinoda, Arata Nagasaka, Ryo Higuchi, Takashi Miura, Yugo Fukazawa4, Kagayaki Kato5, Mayumi Okamoto1, Yasuhiro Inoue, Yoshiaki Minami, Makoto Suzuki, Masaharu Nagayama, Taiji Adachi, Naoto Ueno, Toshihiko Fujimor and Takaki Miyata
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市）
  • Year and Date: 2016-03-28
• [Presentation] Novel mode of action for interkinetic nuclear migration of neural progenitor cell (2015)
  • Author(s): Tomoyasu Shinoda, Arata Nagasaka, Ryo Higuchi, Takashi Miura, Yugo Fukazawa4, Kagayaki Kato5, Mayumi Okamoto1, Yasuhiro Inoue, Yoshiaki Minami, Makoto Suzuki, Masaharu Nagayama, Taiji Adachi, Naoto Ueno, Toshihiko Fujimor and Takaki Miyata
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Novel mode of action for interkinetic nuclear migration of neural progenitor cell (2015)
  • Author(s): Tomoyasu Shinoda, Arata Nagasaka, Ryo Higuchi, Takashi Miura, Yugo Fukazawa4, Kagayaki Kato5, Mayumi Okamoto1, Yasuhiro Inoue, Yoshiaki Minami, Makoto Suzuki, Masaharu Nagayama, Taiji Adachi, Naoto Ueno, Toshihiko Fujimor and Takaki Miyata
  • Organizer: 第48回日本発生生物学学会大会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2015-06-02