Analyses of mutual control mechanism between FMR1 and CPEB1.

• Project/Area Number: 15K18958
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Kansai Medical University
• Principal Investigator: OE Souichi 関西医科大学, 医学部, 助教 (70599331)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: FMR1 / CPEB1 / 脆弱X症候群 / 翻訳制御 / RNA安定性制御

Outline of Final Research Achievements

The aim of this study is elucidation of the mutual translational control mechanism between FMR1, the causative gene of fragile X syndrome, and CPEB1 which acts as a translational repressor. We showed that CPEB co-localized with Fmr1 mRNA in the rat hippocampal neurons and interacted via CPE in the 3 'UTR. Furthermore, knockdown of CPEB1 increased the expression levels of FMR1 and the mitochondria-related genes.

Research Products

• [Journal Article] Mechanism of the Dendritic Translation and Localization of Brain-derived Neurotrophic Factor (2016)
  • Author(s): Oe S, Miki H, Nishimura W, Noda Y.
  • Journal Title: Cell Structure and Function Volume: 41 Issue: 1 Pages: 23-31
  • DOI: 10.1247/csf.15015
  • NAID: 130005139449
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access
• [Presentation] 翻訳抑制因子CPEB1は3’非翻訳領域を介してAuf１によるRNA安定性制御と自己翻訳抑制をうける (2018)
  • Author(s): 大江 総一、田中 進、平原 幸恵、小池 太郎、高森 康晴、山田 久夫
  • Organizer: 第123回日本解剖学会総会・学術総会
• [Presentation] CPEB1は3’UTRを介してFmr1のRNA・タンパク質発現を抑制する (2017)
  • Author(s): 大江 総一、山田 久夫
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学 坂本キャンパス
  • Year and Date: 2017-03-28
• [Presentation] CPEB1 interacts with FMR1 mRNA and regulates its expression levels through 3’ untranslated region. (2017)
  • Author(s): 大江 総一、山田久夫
  • Organizer: 第40回日本神経科学大会
• [Presentation] Cytoplasmic polyadenylation elements and AU-rich elements synergistically regulate CPEB1 mRNA and protein expression during differentiation. (2016)
  • Author(s): 大江 総一、野田 泰子、山田 久夫
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 杏林大学 井の頭キャンパス
  • Year and Date: 2016-09-03
• [Presentation] CPEB1 represses FMR1 mRNA and protein expression through 3’ untranslated region. (2016)
  • Author(s): 大江 総一、山田 久夫
  • Organizer: 第57回組織細胞化学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-07-21
• [Presentation] CPEB1 は3’UTRを介して自身のmRNA樹状突起局在化と局所翻訳を制御する (2015)
  • Author(s): 大江 総一、田中 進、山田 久夫
  • Organizer: 第91 回日本解剖学会・近畿支部学術集会
  • Place of Presentation: 京都工芸繊維大学・総合研究棟
  • Year and Date: 2015-11-28
• [Presentation] 中枢ニューロンにおけるCPEB1 mRNAの樹状突起局在化と翻訳制御 (2015)
  • Author(s): 大江 総一、山田 久夫、三木 玄方、西村 渉、野田 泰子
  • Organizer: 第56回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 関西医科大学枚方キャンパス
  • Year and Date: 2015-10-03