Developmental and pathologic alteration of podocyte 3D structure

• Project/Area Number: 15K18960
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Juntendo University
• Principal Investigator: Ichimura Koichiro 順天堂大学, 医学部, 准教授 (10343485)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 足細胞 / ポドサイト / スリット膜 / 細胞間結合装置 / 三次元超微形態解析 / FIB/SEM / 糸球体発生 / ネフローゼ症候群

Outline of Final Research Achievements

Podocytes are well known to have a unique 3D architecture specialized for glomerular filtration. However, several 3D morphological aspects on podocyte planarization and degeneration remain fragmentary because they are difficult to reveal by conventional scanning electron microscopy. Here, we adopted FIB/SEM tomography, a powerful tool for analyzing the three-dimensional cellular ultrastructure, to precisely reveal the morphological process of podocyte development and the morphological process of podocyte planarization in puromycin aminonucleotide (PAN) nephrosis rats, an animal model for human minimal change nephrotic syndrome.

Research Products

• [Journal Article] Morphological process of podocyte development revealed by block-face scanning electron microscopy (2017)
  • Author(s): Ichimura, K., Kakuta, S., Kawasaki, Y., Miyaki, T., Nonami, T., Miyazaki, N., Enomoto, S., Arai, S., Koike, M., Murata, K., Sakai, T
  • Journal Title: J. Cell Sci. Volume: 130 Pages: 12-23
  • DOI: 10.1242/jcs.187815
  • Peer Reviewed / Open Access
• [Journal Article] Reevaluation of the superior radial collateral artery in the human upper arm. (2017)
  • Author(s): Ichimura K, Kinose S, Kawasaki Y, Kato K, Sakai T
  • Journal Title: Anat Sci Int Volume: (in press) Issue: 1 Pages: 69-74
  • DOI: 10.1007/s12565-016-0368-4
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Structural diversity of the vastus intermedius origin revealed by analysis of isolated muscle specimen. (2017)
  • Author(s): Yoshida S, Ichimura K, Sakai T.
  • Journal Title: Clin Anat Volume: 30(1) Issue: 1 Pages: 98-105
  • DOI: 10.1002/ca.22791
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Novel synthetic compounds with endoperoxide structure damage juvenile stage of Schistosoma mansoni by targeting lysosome-like organelles (2017)
  • Author(s): Yamabe M, Kumagai T, Shimogawara R, Blay EA, Hino A, Ichimura K, Sato A, Kim HS, Ohta N.
  • Journal Title: Parasitol Int Volume: 66(1) Issue: 1 Pages: 917-924
  • DOI: 10.1016/j.parint.2016.10.013
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Electroconvulsive stimulation transiently enhances the permeability of the rat blood-brain barrier and induces astrocytic changes (2017)
  • Author(s): Ito M, Bolati K, Kinjo T, Ichimura K, Furuta A, McLoughlin DM, Suzuki T, Arai H.
  • Journal Title: Brain Res Bull Volume: 128 Pages: 92-97
  • DOI: 10.1016/j.brainresbull.2016.11.011
• [Journal Article] Evolutionary morphology of podocytes and primary urine-producing apparatus (2016)
  • Author(s): ◎Ichimura K, Sakai T.
  • Journal Title: Anat Sci Int Volume: in press Issue: 2 Pages: 161-172
  • DOI: 10.1007/s12565-015-0317-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cathepsin D in podocytes plays an important role in the pathogenesis of proteinuria and chronic kidney disease (2016)
  • Author(s): Nonaka-Yamamoto K, Koike M, Asanuma K, Akiba-Takagi M,1 Trejo JAO, Seki T, Hidaka T, ◎Ichimura K, Sakai T, Tada N, Ueno T, Uchiyama Y, Tomino Y.
  • Journal Title: J Am Soc Nephrol Volume: in press Issue: 9 Pages: 2685-2700
  • DOI: 10.1681/asn.2015040366
  • Peer Reviewed / Open Access
• [Journal Article] Three-dimensional architecture of podocytes revealed by block-face scanning electron microscopy. (2015)
  • Author(s): Ichimura K, Miyazaki N, Sadayama S, Murata K, Koike M, Nakamura K, Ohta K, Sakai T
  • Journal Title: Sci Rep. Volume: 5 Issue: 1 Pages: 8993-8993
  • DOI: 10.1038/srep08993
  • Peer Reviewed / Open Access
• [Journal Article] Podocin is translocated to cytoplasm in puromycin aminonucleoside nephrosis rats and in poor-prognosis patients with IgA nephropathy. (2015)
  • Author(s): Fukuda H, Hidaka T, Takagi-Akiba M, Ichimura K, Trejo JA, Sasaki Y, Wang J, Sakai T, Asanuma K, Tomino Y.
  • Journal Title: Cell & Tissue Research Volume: 360(2) Issue: 2 Pages: 391-400
  • DOI: 10.1007/s00441-014-2100-9
  • Peer Reviewed / Open Access
• [Journal Article] Organ specificity and functional relevance of the arterial structure: a comparative study in the kidney and the skeletal muscle with electron microscopy (2015)
  • Author(s): Hosoyamada Y,◎Ichimura K, Sakai T.
  • Journal Title: J Vasc Res Volume: 52(4) Issue: 4 Pages: 265-272
  • DOI: 10.1159/000442978
  • Peer Reviewed
• [Presentation] 傷害足細胞における突起構造の消失過程 ～ FIB/SEM法を活用した解析 ～ (2017)
  • Author(s): 市村浩一郎、角田宗一郎、宮木貴之、川崎優人、坂井建雄
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎県長崎市 長崎大学医学部
  • Year and Date: 2017-03-28
• [Presentation] 足細胞類縁細胞（ネフロサイト）の超微立体構造 ～ FIB/SEM法を活用した解析 ～ (2017)
  • Author(s): 川崎優人、宮木貴之、角田宗一郎、市村浩一郎、坂井建雄
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎県長崎市 長崎大学医学部
  • Year and Date: 2017-03-28
• [Presentation] 発生期における糸球体内皮細胞の窓形成過程 ～ FIB/SEM法を活用した解析 ～ (2017)
  • Author(s): 細山田康恵、川崎優人、宮木貴之、角田宗一郎、市村浩一郎、坂井建雄
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎県長崎市 長崎大学医学部
  • Year and Date: 2017-03-28
• [Presentation] 糸球体内皮細胞の超微形態 (2017)
  • Author(s): 市村浩一郎、坂井建雄
  • Organizer: 第15回腎病理研究会
  • Place of Presentation: 東京都新宿区 慶應義塾大学医学部
  • Year and Date: 2017-01-07
  • Invited
• [Presentation] 糸球体足細胞における突起とスリット膜の発生過程 ～ SEM連続断面観察法を活用した解析 ～ (2016)
  • Author(s): 市村浩一郎、角田宗一郎、川崎優人、小池正人、坂井建雄
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 福島県郡山市 ビックパレット福島
  • Year and Date: 2016-03-28
• [Presentation] FIB/SEMシステムによる三次元超微形態解析 ～活用事例と有用性～ (2016)
  • Author(s): 市村浩一郎
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: 福島県郡山市 ビックパレット福島
  • Year and Date: 2016-03-28
  • Invited
• [Presentation] SEM連続断面観察法を活用した糸球体足細胞の構造解析 (2015)
  • Author(s): 市村浩一郎、坂井建雄
  • Organizer: 生理研研究会
  • Place of Presentation: 愛知県岡崎市 岡崎コンファレンスセンター
  • Year and Date: 2015-11-19
  • Invited
• [Presentation] SEM連続断面観察法を活用した足細胞の立体構造解析 (2015)
  • Author(s): 市村浩一郎、坂井建雄
  • Organizer: 第47回臨床分子形態学会
  • Place of Presentation: 長崎県長崎市 長崎大学医学部
  • Year and Date: 2015-09-18
  • Invited
• [Presentation] 腎内線維芽細胞のサブグループとその超微形態 (2015)
  • Author(s): 市村浩一郎、坂井建雄
  • Organizer: 第47回臨床分子形態学会
  • Place of Presentation: 長崎県長崎市 長崎大学医学部
  • Year and Date: 2015-09-18
  • Invited