| Project/Area Number |
15K18961
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Juntendo University |
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Principal Investigator |
Sou Yu-shin 順天堂大学, 医学部, 助教 (60576221)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | GPHR / ゴルジ体 / 神経変性 / 小脳回路 / プルキンエ細胞 / バスケット細胞 / 酸性環境 / pH |
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| Outline of Final Research Achievements |
To address the importance of the Golgi lumenal pH homeostasis in brain and neuronal circuit level, we took advantage of GPHR conditional knock-out mice. Brain specific GPHR deficient mice exhibit tremor symptoms, and histological analysis revealed that cerebral cortex and hippocampal CA1 neuron shedding, accumulation of TUNEL positive neurons in the surface layer, fragmentation of Golgi in neuron. These results indicated that brain specific GPHR-deficient mice develop neurodegenerative disease. We also found that disruption of synapse due to axonal degeneration of basket cells and remodeling of neuronal circuits to Purkinje cells occurred in cerebellar inhibitory circuit specific-GPHR deficient mice.
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