Glucose Fluctuations aggravate diabetic cardiomyopathy

• Project/Area Number: 15K18972
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Oita University
• Principal Investigator: Saito Shotaro 大分大学, 医学部, 病院特任助教 (60631380)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 糖尿病 / 血糖変動 / 酸化ストレス / 虚血再灌流障害 / 糖尿病性心筋症 / 洞不全

Outline of Final Research Achievements

Male rats were randomly assigned to either a control, DM, and DM with GF group. DM was induced by an injection of streptozotocin, and glucose fluctuation was induced by starvation and insulin injection. One sequential program comprised 2 hypoglycemic episodes during 4 days. The isolated hearts were subjected to 20-min ischemia/30-min reperfusion. The infarct size was larger in hearts with GF than those with sustained hyperglycemia. Activities of catalase and superoxide dismutase were decreased, and expressions of NADPH oxidase and thioredoxin-interacting protein were upregulated by GF accompanied by an increase of reactive oxygen species (ROS). Swollen mitochondria with destroyed cristae were observed in diabetic hearts; they were further devastated by GF. Microarray analysis revealed that the expressions of miRNA-200c and miRNA-141 were abundant in those hearts with GF.

Research Products

• [Journal Article] Glucose Fluctuations Aggravate Cardiac Susceptibility to Ischemia/Reperfusion Injury by Modulating MicroRNAs Expression (2016)
  • Author(s): Saito S, Thuc LC, Teshima Y, Nakada C, Nishio S, Kondo H, Fukui A, Abe I, Ebata Y, Saikawa T, Moriyama M, Takahashi N.
  • Journal Title: Circulation Journal Volume: 80 Issue: 1 Pages: 186-195
  • DOI: 10.1253/circj.CJ-14-1218
  • NAID: 130005116977
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Presentation] Glucose Fluctuations Aggravate Cardiac Susceptibility to Ischemia/Reperfusion Injury by Modulation MicroRNAs Expression (2017)
  • Author(s): Shotaro Saito
  • Organizer: 第81回日本循環器学会学術集会
  • Place of Presentation: ホテル日航金沢(石川県金沢市)
  • Year and Date: 2017-03-18
  • Invited
• [Presentation] Glucose Fluctuations Impair Cardiac Sinus Node Function in Diabetic Animal Models (2017)
  • Author(s): 池邉 有希
  • Organizer: 第81回日本循環器学会学術集会
  • Place of Presentation: ANAクラウンプラザホテル金沢(石川県金沢市)
  • Year and Date: 2017-03-17