Heteromeric CALHM1/3 in neurotransmission of taste cells

• Project/Area Number: 15K18974
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Kashio Makiko 京都府立医科大学, 医学(系)研究科(研究院), 助教 (20631394)
• Research Collaborator: Taruno Akiyuki 京都府立医科大学, 医学研究科, 講師 (20706824)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 味覚 / ATP / イオンチャネル

Outline of Final Research Achievements

This study has been clarified that recombinant adeno virus vector (AAV-DJ) is an effective gene transfer method to apply in all kinds of taste cell types (type I, II, III) in vivo. The result is expected to largely contribute to the reserch of taste function.

Research Products

• [Journal Article] Subcellular localization of TRPM2 determines the fate of cancer cells, survival or apoptosis (2017)
  • Author(s): Makiko Kashio and Makoto Tominaga
  • Journal Title: Translational Cancer Research Volume: 6 Issue: S2 Pages: S409-S411
  • DOI: 10.21037/tcr.2017.03.09
  • Open Access
• [Journal Article] Adeno-Associated Virus-Mediated Gene Transferinto Taste Cells In Vivo (2017)
  • Author(s): Taruno A, Kashio M, Sun H, Kobayashi K, Sano H, Nambu A, Marunaka Y
  • Journal Title: Chemical Senses Volume: 42 Pages: 69-78
  • DOI: 10.1093/chemse/bjw101
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TRPA1 Channels in Drosophila and Honey Bee Ectoparasitic Mites Share Heat Sensitivity and Temperature-Related Physiological Functions (2016)
  • Author(s): Guangda Peng, Makiko Kashio, Tianbang Li, Xiaofeng Dong, Makoto Tominaga and Tatsuhiko Kadowaki
  • Journal Title: Frontiers in Physiology Volume: 7 Pages: 447-447
  • DOI: 10.3389/fphys.2016.00447
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isoform-specific modulation of chemical sensitivity of conserved TRPA1 channel in a major honey bee ectoparasitic mite, Tropilaelaps mercedesae (2016)
  • Author(s): Xiaofeng Dong, Makiko Kashio, Guangda Peng, Yixuan Lin, Chang Liu1, Sijing Wang, Li Shen, Tiange Li, Makoto Tominaga and Tatsuhiko Kadowaki
  • Journal Title: Open Biol Volume: 6 Issue: 6 Pages: 160042-160042
  • DOI: 10.1098/rsob.160042
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plant-Derived Tick Repellents Activate the Honey Bee Ectoparasitic Mite TRPA1. (2015)
  • Author(s): 1)Guangda Peng*, Makiko Kashio*, Tomomi Morimoto, Tianbang Li, Jingting Zhu, Makoto Tominaga, and Tatsuhiko Kadowaki *Equally contributed.
  • Journal Title: Cell Reports Volume: 12(2) Issue: 2 Pages: 190-202
  • DOI: 10.1016/j.celrep.2015.06.025
  • Peer Reviewed / Open Access
• [Presentation] CALHMチャネル極性ソーティングの分子基盤解析 (2017)
  • Author(s): 加塩麻紀子、樽野陽幸、丸中良典
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: 浜松
• [Presentation] CALHM1チャネルの細胞内局在解析 (2016)
  • Author(s): 5 Mol Cell Biol & Oral Anat, Kyushu Univ, Fukuoka, Japan 加塩 麻紀子、樽野 陽幸、孫 紅昕、小林 憲太、 佐野 裕美、大崎 康吉、城戸 瑞穂、南部 篤、丸中 良典
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2016-03-22
• [Presentation] Adeno-associated virus-mediated gene transduction of taste cells in vivo (2016)
  • Author(s): Makiko Kashio, Akiyuki Taruno, Yoshinori Marunaka
  • Organizer: International Symposium on Olfaction and Taste, JASTS 50th Annual Meeting
  • Place of Presentation: 横浜
  • Int'l Joint Research
• [Presentation] Polarized sorting of CALHM channels in epithelial cells (2016)
  • Author(s): Makiko Kashio, Akiyuki Taruno, Yoshinori Marunaka
  • Organizer: International Symposium on Ion Channels, Transporters and Signal Transduction
  • Place of Presentation: 京都
  • Int'l Joint Research