| Project/Area Number |
15K18976
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Nakajima Hiroyuki 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (10467657)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞内カルシウム / 血管新生 / メカニカルストレス / 血管形成 / 血流 / カルシウム / 血管内皮増殖因子 |
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| Outline of Final Research Achievements |
In this study, we visualized endothelial Ca2+ dynamics at single-cell resolution in zebrafish and found that endothelial Ca2+ oscillations completely depended upon VEGF-A/VEGFR2 signaling during intersomitic vessel (ISV) formation. Thus, visualizing Ca2+ oscillations allowed us to monitor indirectly how VEGF-A/VEGFR2 signaling is spatio-temporally regulated at single cell level. We also observed blood flow-dependent Ca2+ oscillations in perfused vessels. By analyzing endothelial responses to blood flow, we newly found that YAP, a final effector of the Hippo pathway, responds to flow-mediated mechanical stimuli.
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