Adipose insulin signaling regulated by brain KATP channel

• Project/Area Number: 15K18977
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: National Center for Global Health and Medicine
• Principal Investigator: OGURA KOHEI 国立研究開発法人国立国際医療研究センター, 研究所・感染症制御研究部, 上級研究員 (00586612)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: グルコース取り込み / インスリン応答 / KATPチャネル / Akt / 脂肪細胞 / インスリン / カリウムチャネル

Outline of Final Research Achievements

ATP-sensitive potassium (K(ATP)) channel is critical in metabolism control. Previous reports showed that insulin-dependent glucose uptake is enhanced in adipose tissues of KATP channel knockout (KO) mice compared with that in wild-type mice. Insulin stimulated in vivo interaction of Akt and unknown factor in adipose tissues. The interaction was enhanced dependent on amount of injected insulin. Akt2 mainly played role for the interaction in adipose tissues of KO mice. Mass spectrometry analysis showed 14-3-3 family proteins are candidates for the interaction with Akt2.

Research Products

• [Journal Article] Vibrio cholerae Cholix toxin-induced HepG2 cell death is enhanced by tumor necrosis factor-alpha through ROS and intracellular signal-regulated kinases (2017)
  • Author(s): 1.Ogura K, Terasaki Y, Miyoshi-Akiyama T, Terasaki M, Moss J, Noda M, Yahiro K.
  • Journal Title: Toxicological Science Volume: 156 Pages: 455468-455468
  • DOI: 10.1093/toxsci/kfx009
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Acetylation-dependent regulation of MDM2 E3 ligase activity dictates its oncogenic function. (2017)
  • Author(s): 2.Nihira NT, Ogura K, Shimizu K, North BJ, Zhang J, Gao D, Inuzuka H, Wei W.
  • Journal Title: Science Signaling Volume: 10 Issue: 466
  • DOI: 10.1126/scisignal.aai8026
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The SCFβ-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis. (2017)
  • Author(s): Shimizu K, Fukushima H, Ogura K, Lien EC, Nihira NT, Zhang J, North BJ, Guo A, Nagashima K, Nakagawa T, Hoshikawa S, Watahiki A, Okabe K, Yamada A, Toker A, Asara JM, Fukumoto S, Nakayama KI, Nakayama K, Inuzuka H, Wei W.
  • Journal Title: Sci Signal. doi: 10.1126/scisignal.aah4117. Volume: 10 Issue: 460 Pages: 1-15
  • DOI: 10.1126/scisignal.aah4117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Subtilase cytotoxin produced by locus of enterocyte effacement-negative shiga-toxigenic Escherichia coli induces stress granule formation. (2016)
  • Author(s): Tsutsuki H, Yahiro K, Ogura K, Ichimura K, Iyoda S, Ohnishi M, Nagasawa S, Seto K, Moss J, Noda M
  • Journal Title: Cellular Microbiology Volume: in press Issue: 7 Pages: 1024-1040
  • DOI: 10.1111/cmi.12565
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Severe invasive streptococcal infection by Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis. (2016)
  • Author(s): Watanabe S, Takemoto N, Ogura K, Miyoshi-Akiyama T.
  • Journal Title: Microbiology and Immunology Volume: 60 Issue: 1 Pages: 1-9
  • DOI: 10.1111/1348-0421.12334
  • NAID: 40020725365
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] PtdIns(3,4,5)P3-Dependent Activation of the mTORC2 Kinase Complex. (2015)
  • Author(s): Liu P, Gan W, Chin YR, Ogura K, Guo J, Zhang J, Wang B, Blenis J, Cantley LC, Toker A, Su B, Wei W.
  • Journal Title: Cancer Discovery Volume: 5 Issue: 11 Pages: 1194-1209
  • DOI: 10.1158/2159-8290.cd-15-0460
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Studies on relationship between Streptococcus dysgalactiae subsp. equisimilis infection and diabetes mellitus (2017)
  • Author(s): 小倉康平、秋山 徹
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 宮城県仙台市 仙台国際センター
  • Year and Date: 2017-03-19
• [Presentation] コレラ菌由来ADP-リボシル化毒素Cholix toxin による肝細胞致死機構 (2016)
  • Author(s): 小倉康平、寺崎泰弘、秋山 徹、八尋錦之助、野田公俊
  • Organizer: 第63回トキシンシンポジウム
  • Place of Presentation: 山形県天童市 ほほえみの宿 滝の湯
  • Year and Date: 2016-07-14
• [Presentation] Vibrio cholerae Cholix toxin induces hepatocyte cell death through ROS production and MAPK activation. (2016)
  • Author(s): Ogura K, Yahiro K, Miyoshi‐Akiyama T,Noda M
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2016-03-23