| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
T cell is essential for adaptive immunity. T cells is activated by TCR signaling that is indispensable for T cell development and function. I have previously reported that mDia facilitates TCR signaling through the regulation of actin polymerization by utilizing mDia 1/3 double deficient mice. In this study, I further unraveled the molecular mechanism of mDia activation in TCR signaling. We found that mDia is activated by the small G protein RhoA upon TCR stimulation. In addition, using T cells expressing EGFP-mDia3, I found that mDia is localized at the cell periphery in T cells upon TCR stimulation. Therefore, mDia is activated upon TCR stimulation through RhoA at the cell periphery and facilitate TCR signaling through its actin polymerization activity.
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