| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
Palmitoylation is a reversible lipid modification contributing to the regulation of G-protein coupled receptor (GPCR) function. Despite its importance, palmitoylation status of the β3-adrenergic receptor (β3AR), a GPCR critical for lipid metabolism and thermogenesis, has never been determined. We report here that human β3AR is palmitoylated on four cysteine residues at sites in second and third intracellular loop and at two sites in the C-terminal tail. Palmitoylation deficient mutant exhibited lowered cAMP production and ERK1/2 phosphorylation upon agonist stimulation indicating importance for downstream signaling. In addition, less palmitoylated receptor showed altered half-life. These results suggesting that multiple palmitoylations of human β3AR regulate receptor functions.
|
