Development of phospholamban aptamer as a treatment of heart failure for clinical application

Research Project

Project/Area Number	15K18989
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General pharmacology
Research Institution	Yamaguchi University
Principal Investigator	SAKAI Hiroki 山口大学, 医学(系)研究科(研究院), 助教 (40464367)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	心不全治療薬 / アプタマー / 細胞膜透過性ペプチド / ホスホランバン / 核酸アプタマー
Outline of Final Research Achievements	We tried to develop a cell-penetrating phospholamban aptamer as a novel therapeutic tool for treatment of heart failure. We optimized the nucleotide sequence of the phospholamban aptamer which retains the functional activity and is stable in the serum. The obtained phospholamban aptamer enhanced contraction/relaxation of isolated adult rat cardiomyocytes as well as corresponding Ca2+ transients. However, no consistent improvement of cardiac function was obtained in a mouse heart failure model by administration of the aptamer. Further modification would be necessary to improve the pharmacokinetic property of the aptamer for clinical application.

Report

(3 results)