Role of SIRT1 in mitophagy in the skeletal muscle
Project/Area Number |
15K18992
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General pharmacology
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Research Institution | Sapporo Medical University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | マイトファジー / SIRT1 / オートファジー |
Outline of Final Research Achievements |
We found skeletal muscle dysfunction and impaired activation autophagy in skeletal muscle-specific SIRT1 knockout mice. These suggest that SIRT1 in skeletal muscle cells plays an important role in maintaining skeletal muscle function via autophagy. In myoblasts, resveratrol (RSV), a SIRT1 activator, promoted mitophagy. Mitochondrial reactive oxygen species (ROS) levels induced by antimycin A were significantly reduced by RSV. This effect of RSV was blocked by knockdown of PINK-1 which triggers mitophagy, suggesting that SIRT1 activation decrease mitochondrial ROS levels via mitophagy. We demonstrate using in vivo and in vitro models the relationship between SIRT1 and autophagy/mitophagy and its significance in skeletal muscle protection.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Sirt1-deficient mice have hypogonadotropic hypogonadism due to defective GnRH neuronal migration.2015
Author(s)
Di Sante G, Wang L, Wang C, Jiao X, Casimiro MC, Chen K, Pestell TG, Yaman I, Di Rocco A, Sun X, Horio Y, Powell MJ, He X, McBurney MW, Pestell RG.
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Journal Title
Mol Endocrinol
Volume: 29
Issue: 1
Pages: 200-212
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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