| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Glutathione is an important endogenous antioxidant against oxidative stress which is one of the cause of neurodegenerative diseases. Glutathione level has been reported to be regulated by membrane transporter EAAC1 and its negative regulator GTRAP3-18. We have shown that they are regulated by same microRNA although regulatory mechanism for GTRAP3-18 by microRNA is speculated to be mediated by RNA-binding protein. So, we have tried to purify the RNA binding proteins of GTRAP3-18 by magnetic beads bound to RNA sequence of its 3’-UTR. Then we have analyzed the purified proteins by Liquid Chromatography Tandem Mass Spectrometry Method. Combined with the prediction scores from microRNA database, we have obtained six RNA-binding protein candidates of GTRAP3-18. At present, we are trying to identify the RNA-binding protein of GTRAP3-18 using 3’-UTR reporter gene assay and gene knockdown assay.
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