Seeking for target proteins of autophagy related small chemical compounds
| Project/Area Number |
15K19000
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 低分子化合物の標的蛋白質 |
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| Outline of Final Research Achievements |
This study object is to determine target molecules of autophagy related small chemical compounds, such as chloroquine and metformin, and to elucidate the relationships between functions of the molecules and effects of the compounds. TOF-MS analysis revealed the metformin binding protein (MBP) was HMGB1. Metformin inhibited proinflammatory effect of HMGB1 in vitro and in vivo. Furthermore, metformin ameliorated liver injury induced by acetaminophen, as same as anti-HMGB1 antibody. This study elucidated one of an anti-inflammatory target molecule of metformin.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article] Metformin Directly Binds the Alarmin HMGB1 and Inhibits its Proinflammatory Activity.2017
Author(s)
Horiuchi T, Sakata N, Narumi Y, Kimura T, Hayashi T, Nagano K, Liu K, Nishibori M, Tsukita S, Yamada T, Katagiri H, Shirakawa R, Horiuchi H.
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Journal Title
J Biol Chem.
Volume: 印刷中
Issue: 20
Pages: 8436-8446
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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