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Analysis of the mechanism of cytidine deaminase gene regulation using enChIP

Research Project

Project/Area Number 15K19005
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionGifu University

Principal Investigator

SATO Katsuya  岐阜大学, 大学院医学系研究科, 助教 (60733508)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsAID / 遺伝子発現制御 / 転写因子 / エンハンサー領域 / CRISPR/Cas9 / Aicda / 遺伝子転写調節 / 遺伝子発現調節
Outline of Final Research Achievements

Activation-induced cytidine deaminase (AID) is expressed in activated B cells and essential for class switch recombination and somatic hypermutation. In addition, it is reported that AID can be involved in tumor progression and epigenetic regulation even in the non-B cells. To date, at least 11 transcription factors and 6 cis-regulatory elements have been identified as regulators of Aicda, the gene of AID. However, the detailed mechanisms to limited the AID expression in activated B cells are not fully understood. In this study, we investigated the mechanism of Aicda regulation.
We revealed that Batf plays an essential role and the binding ability of BATF and IRF4 are important for AID expression. We established the cells that harboring deletion of each Aicda cis-regulatory element and found the Batf-IRF4 complex binds to a region different from the reported region on Aicda. Also, we examined the binding site of the complex and DNA structure-to-function relationship by ChIP and enChIP.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (12 results)

  • [Journal Article] Specific activation of PLEKHG2-induced serum response element-dependent gene transcription by four-and-a-half LIM domains (FHL) 1, but not FHL2 or FHL32017

    • Author(s)
      Masashi Nishikawa, Katsuya Sato, Shun Nakano, Hisashi Yamakawa, Takahiro Nagase, Hiroshi Ueda
    • Journal Title

      Small GTPases,

      Volume: 10 Issue: 5 Pages: 1-6

    • DOI

      10.1080/21541248.2017.1327838

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heterotrimeric G protein Galphas subunit attenuates PLEKHG2, a Rho family-specific guanine nucleotide exchange factor, by direct interaction2017

    • Author(s)
      Sugiyama K, Tago K, Matsushita S, Nishikawa M, Sato K, Muto Y, Nagase T, Ueda H
    • Journal Title

      Cell Signal

      Volume: 32 Pages: 115-123

    • DOI

      10.1016/j.cellsig.2017.01.022

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] M2-like macrophage polarization in high lactic acid-producing head and neck cancer.2017

    • Author(s)
      Ohashi, T, Aoki, M, Tomita, H, Akazawa, T, Sato, K, Kuze, B, Mizuta, K, Hara, A, Nagaoka, H, Inoue, N, Ito, Y
    • Journal Title

      Cancer Sci

      Volume: 印刷中 Issue: 6 Pages: 1128-1134

    • DOI

      10.1111/cas.13244

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis.2016

    • Author(s)
      Sato, K., Kimura, M., Sugiyama, K., Nishikawa, M., Okano, Y., Nagaoka, H., Nagase, T., Kitade, Y., and Ueda, H.
    • Journal Title

      J Biol Chem

      Volume: 291 Issue: 48 Pages: 25227-25238

    • DOI

      10.1074/jbc.m116.759571

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 抗体遺伝子改変酵素AIDの発現制御に関わる転写調節領域の解析2017

    • Author(s)
      佐藤克哉、金山佳史、堀賢一郎、安田一、長岡仁
    • Organizer
      第81回日本生化学会中部支部例会・シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] 抗体遺伝子改変酵素AIDの遺伝子発現調節におけるシス調節エレメントの解析2017

    • Author(s)
      安田 一、佐藤 克哉、金山 佳史、堀 賢一郎、長岡 仁
    • Organizer
      第49回日本臨床分子形態学会総会・学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 非受容体型チロシンキナーゼABL1によるRho活性化因子PLEKHG2の活性制御と核内移行2017

    • Author(s)
      西川 将司、中野 駿、中尾 拡、佐藤 克哉、山川 央、長瀬 隆弘、上田 浩
    • Organizer
      生命科学系合同年次大会・第40回日本分子生物学会/第90回日本生化学会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] EPHB2/SRCシグナルを介したRho活性化因子DBSのチロシンリン酸化2017

    • Author(s)
      中野 駿、西川 将司、浅岡 里奈、田代 圭、石川 奈津子、大脇 千智、佐藤 克哉、山川 央、 長瀬 隆弘、上田 浩
    • Organizer
      生命科学系合同年次大会・第40回日本分子生物学会/第90回日本生化学会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CRISPR/Cas9によるシス調節エレメントの編集を用いた抗体遺伝子改変酵素AIDの発現調節機構の解析2017

    • Author(s)
      佐藤 克哉、安田 一、金山 佳史、堀 賢一郎、長岡 仁
    • Organizer
      生命科学系合同年次大会・第40回日本分子生物学会/第90回日本生化学会合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analysis of cis-regulatory elements involved in the regulation of expression of Aicda2017

    • Author(s)
      Katsuya Sato、Hitoshi Nagaoka
    • Organizer
      第46回日本免疫学会総会・学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 三量体G蛋白質によるRho活性化因子PLEKHG1の活性制御2016

    • Author(s)
      西川将司、杉山和恵、佐藤克哉、山川央、長瀬隆弘、上田浩
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Rho活性化因子PLEKHG2のFour and a half LIMドメイン含有蛋白質との相互作用による活性制御2016

    • Author(s)
      佐藤克哉、木村正志、杉山和恵、岡野幸雄、長岡仁、長瀬隆弘、北出幸夫、上田浩
    • Organizer
      第80回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      三重大学
    • Related Report
      2016 Research-status Report
  • [Presentation] 三量体G蛋白質によるRho活性化因子PLEKHG1の活性制御2016

    • Author(s)
      西川将司、杉山和恵、佐藤克哉、長瀬隆弘、上田浩
    • Organizer
      日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2016
    • Place of Presentation
      長良川国際会議場・岐阜都ホテル
    • Related Report
      2016 Research-status Report
  • [Presentation] CRISPR/Cas9を用いた抗体遺伝子改変酵素AIDの発現制御機構の解析2015

    • Author(s)
      堀賢一郎、佐藤克哉、金山佳史、安田一、長屋州宣、長岡仁
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会 合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Evaluation of function of transcription factors, Batf and HoxC4, in Aicda gene regulation2015

    • Author(s)
      佐藤克哉、長岡仁
    • Organizer
      第44回日本免疫学会総会・学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 抗体遺伝子改変酵素AIDの発現制御機構の解析2015

    • Author(s)
      金山佳史、佐藤克哉、堀賢一郎、長岡仁
    • Organizer
      第79回日本生化学会中部支部例会・シンポジウム
    • Place of Presentation
      松本
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report

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Published: 2015-04-16   Modified: 2019-03-29  

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