| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Activation-induced cytidine deaminase (AID) is expressed in activated B cells and essential for class switch recombination and somatic hypermutation. In addition, it is reported that AID can be involved in tumor progression and epigenetic regulation even in the non-B cells. To date, at least 11 transcription factors and 6 cis-regulatory elements have been identified as regulators of Aicda, the gene of AID. However, the detailed mechanisms to limited the AID expression in activated B cells are not fully understood. In this study, we investigated the mechanism of Aicda regulation.
We revealed that Batf plays an essential role and the binding ability of BATF and IRF4 are important for AID expression. We established the cells that harboring deletion of each Aicda cis-regulatory element and found the Batf-IRF4 complex binds to a region different from the reported region on Aicda. Also, we examined the binding site of the complex and DNA structure-to-function relationship by ChIP and enChIP.
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