Study of microglial transformation mechanism for ALS therapy

• Project/Area Number: 15K19006
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Nagoya University
• Principal Investigator: Kazuyoshi Kobayashi 名古屋大学, 医学部附属病院, 病院助教 (00706294)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 脊髄損傷 / ミクログリア / ケラタン硫酸 / ALS / 脊髄変性疾患 / 形質転換 / 脊髄変性 / 運動神経

Outline of Final Research Achievements

We elucidated the mechanism of keratan sulfate involvement in ALS pathogenesis. Increased expression of chemokines (CCL 2, CCL 3, CCL 4, CC 5, CCL 7, CCL 12) was observed in the KSPG deficient mouse group. In addition, upregulation of ligand / receptor related to GAG binding was identified. Chemokines are basic proteins that exert their effects through G protein-coupled receptors. Then, chemokines cause migration such as white blood cells and are involved in the formation of inflammation. In summary, GAG binding due to the presence of KSPG was thought to be involved in the onset of spinal degeneration.

Research Products

• [Journal Article] Contrast MRI Findings for Spinal Schwannoma as Predictors of Tumor Proliferation and Motor Status. (2017)
  • Author(s): Kobayashi K, Imagama S, Ando K, Hida T, Ito K, Tsushima M, Ishikawa Y, Matsumoto A, Morozumi M, Tanaka S, Ishiguro N.
  • Journal Title: Spine Volume: 42(3)
  • NAID: 120005972925
  • Peer Reviewed
• [Journal Article] Prevention of spinal cord injury using brain-evoked muscle-action potential (Br(E)-MsEP) monitoring in cervical spinal screw fixation. (2017)
  • Author(s): Kobayashi K, Imagama S, Ito Z, Ando K, Hida T, Ishiguro N.
  • Journal Title: Eur Spine J Volume: - Issue: 4 Pages: 1154-1161
  • DOI: 10.1007/s00586-016-4941-2
  • Peer Reviewed
• [Journal Article] Analysis of Incident and Accident Reports and Risk Management in Spine Surgery. (2017)
  • Author(s): Kobayashi K, Imagama S, Ando K, Hida T, Ito K, Tsushima M, Ishikawa Y, Matsumoto A, Morozumi M, Nishida Y, Nagao Y, Ishiguro N.
  • Journal Title: Spine Volume: - Issue: 15 Pages: 1184-1188
  • DOI: 10.1097/brs.0000000000002072
  • Peer Reviewed
• [Journal Article] Collaboration with an infection control team for patients with infection after spine surgery. (2017)
  • Author(s): Kobayashi K, Imagama S, Kato D, Ando K, Hida T, Ito K, Tsushima M, Matsumoto A, Morozumi M, Tanaka S, Yagi T, Nishida Y, Ishiguro N.
  • Journal Title: Am J Infect Control Volume: 6553(17) Pages: 30035-4
  • Peer Reviewed
• [Journal Article] Is a Drain Tip Culture Required After Spinal Surgery? (2016)
  • Author(s): Kobayashi K,Imagama S, Ito Z, Ando K, Yagi H, Hida T, Ito K, Ishikawa Y, Tsushima M, Ishiguro N.
  • Journal Title: Clin Spine Surg Volume: -
  • NAID: 120006491434
  • Peer Reviewed
• [Journal Article] Tuberculous meningitis with dementia as the presenting symptom after intramedullary spinal cord tumor resection (2015)
  • Author(s): Kobayashi K, Imagama S, Ito Z, Ando K, Yagi H, Shinjo R, Hida T, Ito K, Ishikawa Y, Matsuyama Y, Ishiguro N.
  • Journal Title: Nagoya J. Med. Sci Volume: 77 Pages: 653-657
  • NAID: 120005672318
  • Peer Reviewed
• [Journal Article] Utility of a 3-dimensional full-scale NaCl model for rib strut grafting for anterior fusion for cervicothoracic kyphosis (2015)
  • Author(s): Kobayashi K, Imagama S, Muramoto A, Ito Z, Ando K, Yagi H, Hida T, Ito K, Ishikawa Y, Tsushima M, Ishiguro N.
  • Journal Title: Nagoya J. Med. Sci Volume: 77 Pages: 491-496
  • NAID: 120005650124
  • Peer Reviewed
• [Journal Article] Fatal case of cervical blunt vascular injury with cervical vertebral fracture: A case report (2015)
  • Author(s): Kobayashi K, Imagama S, Okura T, Yoshihara H, Ito Z, Ando K, Ukai J, Shinjo R, Muramoto A, Matsumoto T, Nakashima H, Ishiguro N.
  • Journal Title: Nagoya J. Med. Sci Volume: 77 Pages: 473-480
  • NAID: 120005650121
  • Peer Reviewed
• [Journal Article] Spinal metastasis from struma ovarii: case report and review of the literature (2015)
  • Author(s): Kobayashi K, Imagama S, Tsunekawa S, Hosokawa K, Watanabe M, Ito Z, Ando K, Ishiguro N.
  • Journal Title: Asian Spine J Volume: 9 Issue: 2 Pages: 281-285
  • DOI: 10.4184/asj.2015.9.2.281
  • NAID: 120005946846
  • Peer Reviewed
• [Journal Article] Hyperamylasemia and pancreatitis following posterior spinal surgery (2015)
  • Author(s): Kobayashi K, Imagama S, Ito Z, Ando K, Shinjo R, Yagi H, Hida T, Ito K, Ishikawa Y, Ishiguro N.
  • Journal Title: J Orthop Sci Volume: 20 Issue: 6 Pages: 967-972
  • DOI: 10.1007/s00776-015-0754-0
  • NAID: 120005972926
  • Peer Reviewed
• [Presentation] 80 歳以上に行った脊椎手術の周術期合併症の発生率とリスク因子の検討 (2016)
  • Author(s): 小林和克・今釜史郎・安藤圭・石黒直樹
  • Organizer: 日本整形外科学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] 胸椎に行った菅笠式椎弓形成の有用性 (2016)
  • Author(s): 小林和克・今釜史郎・安藤圭・石黒直樹
  • Organizer: 日本整形外科学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] 脊椎術後に生じた重大事象例の検討 (2016)
  • Author(s): 小林和克・今釜史郎・安藤圭・石黒直樹
  • Organizer: 日本整形外科学会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-05-12
  • Invited
• [Presentation] Consultation with the Infection Control Team for cases with infection after spinal surgery (2016)
  • Author(s): 小林和克・今釜史郎・伊藤全哉・安藤圭・石黒直樹
  • Organizer: 日本脊椎脊髄病学会
  • Place of Presentation: 幕張メッセ国際会議場（千葉県千葉市）
  • Year and Date: 2016-04-13
  • Int'l Joint Research / Invited
• [Presentation] Study of hyperamylasemia and pancreatitis following spinal surgery (2016)
  • Author(s): Kazuyoshi Kobayashi
  • Organizer: IMAST
  • Place of Presentation: 米国オーランド
  • Year and Date: 2016-03-02
  • Int'l Joint Research
• [Presentation] Is a drain tip culture after spinal surgery necessary? (2015)
  • Author(s): Kazuyoshi Kobayashi
  • Organizer: AAOS(米国整形外科学会)
  • Place of Presentation: マレーシア クアラルンプール
  • Year and Date: 2015-07-08
  • Int'l Joint Research
• [Book] 脊髄腫瘍の臨床 (2016)
  • Author(s): 小林和克・見松健太郎・松山幸弘
  • Total Pages: 231
  • Publisher: メディカ出版
• [Book] 整形外科 (vol.66, no.10) (2015)
  • Author(s): 小林和克
  • Publisher: 南江堂