Elucidating the mechanisms underlying linear ubiquitin chains-mediated cell death protection
Project/Area Number |
15K19007
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Kyoto University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 細胞死 / LUBAC / 直鎖状ポリユビキチン鎖 |
Outline of Final Research Achievements |
LUBAC is involved in TNF-alpha mediated NF-kappaB activation via generating linear polyubiquitin chains. We discovered that LUBAC is also involved in the protection of TNF-alpha-mediated cell death in NF-kappaB independent manner. To elucidate the mechanisms, I prepared LUBAC knockout cells and reconstituted the several LUBAC mutants into cells to analyze whose domains are involved in cell death protection. Finally, we found a domain of LUBAC which participates in cell death regulation and explored proteins which bind to newly discovered domain by Mass spectrometry.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Crystal structure of the N-myristoylated lipopeptide-bound MHC class I complex.2015
Author(s)
Morita D, Yamamoto Y, Mizutani T, Ishikawa T, Suzuki J, Igarashi T, Mori N, Shiina T, Inoko H, Fujita H, Iwai K, Tanaka Y, Mikami B, Sugita M,
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Journal Title
Nat Commun.
Volume: 7
Issue: 1
Pages: 10356-10356
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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