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Molecular mechanisms for plasma membrane damage-dependent cellular senescence

Research Project

Project/Area Number 15K19012
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionNagoya City University

Principal Investigator

KONO Keiko  名古屋市立大学, 大学院医学研究科, 講師 (30632723)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords細胞創傷治癒 / 細胞老化 / 老化 / 出芽酵母 / ストレス / 脂質
Outline of Final Research Achievements

We found that physical damage to the plasma membrane induces the formation of "scars", membrane projections with altered lipid composition, after plasma membrane repair in budding yeast and human cultured cells. Genetic manipulation that removes the scars significantly extended yeast replicative lifespan. These results suggest that the scar itself, or the altered phospholipid composition, seems to be a cause for cellular senescence after plasma membrane damage. During the course of these studies, we unexpectedly discovered a previously unappreciated cell cycle checkpoint that senses plasma membrane damage. The "plasma membrane damage checkpoint" transiently arrests the cell cycle in yeasts and human cultured cells.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results)

  • [Int'l Joint Research] Brooklyn college(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Brooklyn Collage/Brown University(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Ypk1/Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses.2017

    • Author(s)
      Hatakeyama R, Kono K and Yoshida S.
    • Journal Title

      J. Cell. Sci.

      Volume: 130 Issue: 6 Pages: 1169-1178

    • DOI

      10.1242/jcs.198382

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A new cell cycle checkpoint that senses plasma membrane/cell wall damage in budding yeast.2017

    • Author(s)
      Kono K and Ikui AE.
    • Journal Title

      BioEssays.

      Volume: 39 Issue: 4 Pages: 1600210-1600210

    • DOI

      10.1002/bies.201600210

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Examination and Disruption of the Yeast Cell Wall2016

    • Author(s)
      Okada H, Kono K, Neiman AM, Ohya Y
    • Journal Title

      Cold Spring Harb Protoc.

      Volume: 2016 Issue: 8 Pages: 078659-078659

    • DOI

      10.1101/pdb.top078659

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Local and Acute Disruption of the Yeast Cell Surface2016

    • Author(s)
      Kono K, Okada H, Ohya Y
    • Journal Title

      Cold Spring Harb Protoc.

      Volume: 2016 Issue: 8 Pages: pdb.prot085266-pdb.prot085266

    • DOI

      10.1101/pdb.prot085266

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint during G1 in S. cerevisiae.2016

    • Author(s)
      Kono K, Al-Zain A, Schroeder L, Nakanishi M, Ikui AE.
    • Journal Title

      Proc. Natl. Acad, Sci. U. S. A.

      Volume: 113 Issue: 25 Pages: 6910-6915

    • DOI

      10.1073/pnas.1523824113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plasma membrane/cell wall perturbation activates a novel cell cycle checkpoint during G1 in S. cerevisiae2016

    • Author(s)
      Kono K, Al-Zain A, Schroeder L, Nakanishi M, Ikui AE
    • Journal Title

      Proc. Natl. Acad. Sci.

      Volume: N.D.

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] PP1-Dependent Formin Bnr1 Dephosphorylation and Delocalization from a Cell Division Site2016

    • Author(s)
      Orii M, Kono K, Wen HI, Nakanishi M.
    • Journal Title

      PLOS ONE

      Volume: 11 Issue: 1 Pages: e0146941-e0146941

    • DOI

      10.1371/journal.pone.0146941

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 細胞創傷治癒 その分子機構と老化との関連2015

    • Author(s)
      河野恵子
    • Journal Title

      化学と生物

      Volume: 53 Pages: 751-755

    • NAID

      130005431476

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 細胞創傷治癒 その分子機構と老化との関連2017

    • Author(s)
      河野恵子
    • Organizer
      第1回産学連携21世紀酵母フォーラム
    • Place of Presentation
      サントリーグローバルイノベーションセンター(京都府相楽郡)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 細胞膜損傷は分裂寿命を短縮する2016

    • Author(s)
      河野恵子、西村耕太郎、城村由和、森山陽介、佐藤良勝、 東山哲也、中西真
    • Organizer
      酵母遺伝学フォーラム第49回研究報告会
    • Place of Presentation
      シーサイドホテル舞子ビラ (兵庫県神戸市)
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞創傷治癒  -その分子機構と老化との関連-2016

    • Author(s)
      河野恵子
    • Organizer
      広島大学健康長寿拠点セミナーシリーズ
    • Place of Presentation
      広島大学 (広島県 東広島市)
    • Year and Date
      2016-01-22
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 酵母で明らかにする新しい老化のメカニズム2016

    • Author(s)
      河野恵子
    • Organizer
      酵母ルネッサンス2
    • Place of Presentation
      東京大学先端科学技術研究センター(東京都目黒区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 細胞創傷治癒 その分子機構と老化との関連2016

    • Author(s)
      河野恵子
    • Organizer
      生理化学研究ユニット第6回シンポジウム
    • Place of Presentation
      京都大学農学部大講義室(京都府京都市左京区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Cellular wound healing checkpoint inhibits DNA replication and polarized cell growth2015

    • Author(s)
      Amy Ikui, Keiko Kono
    • Organizer
      Cold Spring Harbor Yeast Cell Biology Meeting
    • Place of Presentation
      New York (USA)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2022-02-16  

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