| Project/Area Number |
15K19013
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Iwate Medical University (2016-2017)
Wakayama Medical University (2015) |
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Principal Investigator |
Murashima Aki 岩手医科大学, 医学部, 助教 (50637105)
|
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| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 中腎 / 尿管芽 / beta-catenin / Fgf / 遺伝子改変マウス / MAPKシグナル / 腎臓発生 / 細胞増殖因子 / ウォルフ管 |
|---|
| Outline of Final Research Achievements |
Hoxb7-Cre mediated removal of beta-catenin from the mouse Wolffian duct (WD) epithelium results in the ectopic ureteric bud (UB) formation. The expression of GDNF/Ret and its intracellular signaling pathways, PI3K/Akt and MEK/Erk, which are important for the normal UB formation, were not augmented in such ectopic ureteric buds. While prominent c-Jun phosphorylation was observed indicating GDNF/Ret signaling independent activation of MAPK signaling pathway during UB formation. Genetic ablation of Fgfr2 in the background of WD epithelia-specific beta-catenin KO did not rescue the phenotype. These results indicate that the presence of novel signaling pathway, other than GDNF/Ret, FGF and BMP, for the UB formation giving better understanding for human congenital anomalies, e.g. CAKUT.
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