• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of GATA2 in mast cells

Research Project

Project/Area Number 15K19014
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionTakasaki University of Health and Welfare

Principal Investigator

Ohmori Shinya  高崎健康福祉大学, 薬学部, 助教 (10509194)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsGATA2 / mast cells / トランスジェニックマウス / BAC / In vivo / BAC-TG / マスト細胞 / Cebpa / 形質転換
Outline of Final Research Achievements

We previously reported that ablation of GATA2 in mouse bone marrow-derived mast cells (BMMCs) resulted in the dedifferentiation of mast cells into myeloid-like cells. In this study, we examined whether this phenomenon is also observed in vivo. Firstly, the tdTomato(+)GATA2-/-BMMCs were transplanted into the peritoneal cavity of wild type mice. Unexpectedly, however, tdTomato-positive cells were not detectable in the mesentery and peritoneal cavity of the mice 7 days following the transplantation. Secondly, we generated BAC transgenic mice harboring mast cell-specific CreERT2 and obtained 6 lines of F1 mice. Transgene copy number was estimated for 1-3 by quantitative genomic PCR. However, none of these lines express CreERT2 transgene in their peritoneal mast cells. Collectively, alternative strategies might be considered for studying GATA2-deficient mast cells in vivo.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015

All Journal Article (3 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] Different Regulation of P-Glycoprotein Function between Caco-2 and Caki-1 cells by ERM proteins2016

    • Author(s)
      Yano K, Otsuka K, Kato Y, Kawabata H, Ohmori S, Arakawa H, Ogihara T.
    • Journal Title

      J Pharm Pharmacol.

      Volume: 68 Issue: 3 Pages: 361-367

    • DOI

      10.1111/jphp.12525

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] GATA2 is critical for the maintenance of cellular identity in differentiated mast cells derived from mouse bone marrow.2015

    • Author(s)
      Ohmori S, Moriguchi T, Noguchi Y, Ikeda M, Kobayashi K, Tomaru N, Ishijima Y, Ohneda O, Yamamoto M, Ohneda K.
    • Journal Title

      Blood

      Volume: 125 Issue: 21 Pages: 3306-3315

    • DOI

      10.1182/blood-2014-11-612465

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] GATA2によるマスト細胞の分化と維持2015

    • Author(s)
      大森慎也, 大根田絹子
    • Journal Title

      臨床免疫・アレルギー科

      Volume: 64 Pages: 352-365

    • Related Report
      2015 Research-status Report
  • [Presentation] マウス脂肪組織由来脂肪前駆細胞の分化過程における転写因子GATA2の機能解析2017

    • Author(s)
      大森慎也, 和田圭祐, 鈴木美穂, 風間由紀子, 石島康史, 大根田絹子
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] 3T3-L1細胞の脂肪細胞分化においてGlucocorticoid受容体の活性化がGATA2の発現抑制をもたらす2016

    • Author(s)
      石嶋康史, 大森慎也, 采女愛, 青木佑介, 小堀美樹, 大根田絹子
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] マウス骨髄由来マストにおけるGATA2、PU.1、RUNX1によるCebpa抑制メカニズムの解析2016

    • Author(s)
      大森慎也, 掛野晶, 石嶋康史, 大根田絹子
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] マスト細胞におけるGATA2のCebpa転写抑制メカニズムの解析-CRISPR/Cas9法によるCebpa+37K領域の機能的貢献の解析2016

    • Author(s)
      掛野晶, 大森慎也, 石嶋康史, 大根田絹子
    • Organizer
      平成28年度日本生化学会関東支部例会―生命科学に基づく診断と治療―
    • Place of Presentation
      自治医科大学(栃木県下野市)
    • Year and Date
      2016-06-11
    • Related Report
      2016 Research-status Report
  • [Presentation] マスト細胞におけるGATA2のCebpa転写抑制メカニズムの解析ーCRISPR/Cas9法によるCebpa+37K領域の機能的貢献の解析ー2016

    • Author(s)
      掛野晶, 大森慎也, 石嶋康史, 大根田絹子
    • Organizer
      平成28年度 日本生化学会関東支部例会
    • Place of Presentation
      自治医科大学 医学部(栃木県下野市)
    • Year and Date
      2016-06-11
    • Related Report
      2015 Research-status Report
  • [Presentation] マスト細胞分化過程におけるGATA2を介したCebpa遺伝子の発現抑制メカニズムの解析2015

    • Author(s)
      大森慎也, 石嶋康史, 大根田絹子
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 脂肪細胞分化におけるGATA因子の発現抑制機構の解析2015

    • Author(s)
      石嶋康史, 大森慎也, 青木祐介, 采女愛, 丹野志保, 前川悠理, 大根田絹子
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] マスト細胞分化におけるGATA2の機能と役割2015

    • Author(s)
      大森慎也
    • Organizer
      第11回 麒麟塾(血液学若手研究者勉強会)
    • Place of Presentation
      コクヨホール(東京都港区)
    • Year and Date
      2015-07-11
    • Related Report
      2015 Research-status Report
    • Invited

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi