Functional analysis of GATA2 in mast cells
| Project/Area Number |
15K19014
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Takasaki University of Health and Welfare |
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Principal Investigator |
Ohmori Shinya 高崎健康福祉大学, 薬学部, 助教 (10509194)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | GATA2 / mast cells / トランスジェニックマウス / BAC / In vivo / BAC-TG / マスト細胞 / Cebpa / 形質転換 |
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| Outline of Final Research Achievements |
We previously reported that ablation of GATA2 in mouse bone marrow-derived mast cells (BMMCs) resulted in the dedifferentiation of mast cells into myeloid-like cells. In this study, we examined whether this phenomenon is also observed in vivo. Firstly, the tdTomato(+)GATA2-/-BMMCs were transplanted into the peritoneal cavity of wild type mice. Unexpectedly, however, tdTomato-positive cells were not detectable in the mesentery and peritoneal cavity of the mice 7 days following the transplantation. Secondly, we generated BAC transgenic mice harboring mast cell-specific CreERT2 and obtained 6 lines of F1 mice. Transgene copy number was estimated for 1-3 by quantitative genomic PCR. However, none of these lines express CreERT2 transgene in their peritoneal mast cells. Collectively, alternative strategies might be considered for studying GATA2-deficient mast cells in vivo.
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Report
(4 results)
Research Products
(11 results)
