Phosphorylation of SWI/SNF chromatin remodeling component Brg1 and mechanisms involved in malignant transformation of cancer
| Project/Area Number |
15K19028
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Yokohama City University |
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Principal Investigator |
KIMURA Ayuko 横浜市立大学, 先端医科学研究センター, 特任助教 (50553616)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | リン酸化 / 癌 / 悪性化 / プロテオミクス / クロマチン再構成複合体 / 卵巣明細胞癌 / 悪性度 / SWI/SNF複合体 / Brg1 / OCCC / 卵巣癌 / SWI/SNF / 質量分析 |
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| Outline of Final Research Achievements |
The SWI/SNF complex contains many tumor suppressors (e.g., ARID1A and BRG1) that are frequently mutated in cancers including ovarian clear cell carcinoma (OCCC), a malignant subtype of ovarian cancers. Previously, we reported that the phosphorylation of BRG1 was significantly reduced in OCCC cells. To reveal the role of BRG1 phosphorylation, we analyzed the transcriptome, proteome, and phenotypes of cell lines with or without mutations in the phosphorylation site.
Proteome analysis revealed the upregulation of proteins and phosphoproteins involved in chromatin inactivation in phosphorylation-mimic mutant. In addition, the proliferative and migratory abilities of phosphorylation-mimic cells was decreased as compared to those of wild-type or phosphorylation-deleted cells. Transcriptome analysis also revealed their quantitative differences in genes involved in cell proliferation and migration, indicating the role of BRG1 phosphorylation in regulation of these genes.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Lyn Kinase Suppresses the Transcriptional Activity of IRF5 in the TLR-MyD88 Pathway to Restrain the Development of Autoimmunity.2016
Author(s)
Ban T., Sato G.R., Nishiyama A., Akiyama A., Takasuna M., Umehara M., Suzuki S., Ichino M., Matsunaga S., Kimura A., Kimura Y., Yanai H., Miyashita S., Kuromitsu J., Tsukahara K., Yoshimatsu K., Endo I., Yamamoto T., Hirano H., Ryo A., Taniguchi T., Tamura T.
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Journal Title
Immunity
Volume: 45
Issue: 2
Pages: 319-332
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Comprehensive behavioral study and proteomic analyses of CRMP2-deficient mice.2016
Author(s)
Nakamura H, Yamashita N, Kimura A, Kimura Y, Hirano H, Makihara H, Kawamoto Y, Jitsuki-Takahashi A, Yonezaki K, Takase K, Miyazaki T, Nakamura F, Tanaka F, Goshima Y.
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Journal Title
Genes to Cells
Volume: 21
Issue: 10
Pages: 1059-1079
DOI
Related Report
Peer Reviewed / Open Access
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