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Involvement of the type of PCSK9 in familial hypercholesterolemia, obesity and diabetes

Research Project

Project/Area Number 15K19038
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pathological medical chemistry
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

Mika Hori  国立研究開発法人国立循環器病研究センター, 研究所, 室長 (60598043)

Project Period (FY) 2015-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsPCSK9 / 変異 / 分子型 / LDL-コレステロール / 家族性高コレステロール血症 / バリアント
Outline of Final Research Achievements

In familial hypercholesterolemia, there were no significant differences in serum lipid levels and the prevalence of coronary artery diseases (CADs) between PCSK9 V4I carriers and non-carriers without LDLR mutations. In the patients carrying LDLR mutations and aged 30 years or more, the additional PCSK9 V4I variant is linked to a significantly increased prevalence of CADs in accord with the elevation of the LDL-C level. In vitro experiments, there were no differences in the expression of LDLR and the uptake of LDL between PCSK9 V4I-expressed cells and PCSK9 WT-expressed cells. However, in a setting, the uptake of LDL was significantly decreased in PCSK9 V4I-expressed cells as compared with PCSK9 WT- expressed cells.
In addition, there was inverse correlation between ratio of furin-cleaved PCSK9 to total PCSK9 and serum APOB level in patients with hypercholesterolemia. Thus, it is suggested that the rate of mature PCSK9 to total PCSK9 is associated with high LDL-C levels.

Report

(3 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2015

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Mature proprotein convertase subxilisin/kexin Type 9, coronary atheroma burden and vessel remodeling in heterozygous familial hypercholesterolemia2017

    • Author(s)
      Kataoka Y, Harada-Shiba M, Nakao K, Nakashima T, Kawakami S, Fujino M, Kanaya T, Nagai T, Tahara Y, Asaumi Y, Hori M, Ogura M, Goto Y, Noguchi T, Yasuda S
    • Journal Title

      Journal of Clinical Lipidology

      Volume: 11 Issue: 2 Pages: 413-421

    • DOI

      10.1016/j.jacl.2017.01.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proprotein convertase subtilisin/kexin 9 (PCSK9) V4I variant with LDLR mutations modifies the phenotype of familial hypercholesterolemia2016

    • Author(s)
      Ohta N*, Hori M*†, Takahashi A, Ogura M, Makino H, Tamanaha T, Fujiyama H, Miyamoto Y, Harada-Shiba M†(*equal contribution, †corresponding author)
    • Journal Title

      Journal of Clinical Lipidology

      Volume: 印刷中 Issue: 3 Pages: 547-555.e5

    • DOI

      10.1016/j.jacl.2015.12.024

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Association between Cholesterol Efflux Capacity and Atherosclerotic Cardiovascular Disease in Patients with Familial Hypercholesterolemia2016

    • Author(s)
      Masatsune Ogura, Mika Hori, Mariko Harada-Shiba.
    • Journal Title

      Arterioscler Thromb Vasc Biol.

      Volume: 36(1) Issue: 1 Pages: 181-188

    • DOI

      10.1161/atvbaha.115.306665

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of pancreatic fatty infiltration in pancreatic carcinogenesis2016

    • Author(s)
      Hori M, Mutoh M, Imai T, Nakagama H
    • Journal Title

      Journal of the Pancreas

      Volume: 17 Pages: 166-175

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Proprotein convertase subtilisin/kexin 9 (PCSK9) V4I variant with LDLR mutations modifies the phenotype of heterozygous familial hypercholesterolemia2016

    • Author(s)
      Mika Hori, Naotaka Ohta, Atsushi Takahashi, Masatsune Ogura, Hisashi Makino, Tamiko Tamanaha, Yoshihiro Miyamoto, Mariko Harada-Shiba
    • Organizer
      84th European Atherosclerosis Society Congress
    • Place of Presentation
      インスブルック、オーストリア
    • Year and Date
      2016-05-31
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] LDLアフェレシスにより除去されるタンパク質の解析ーFHと末梢動脈疾患ー2016

    • Author(s)
      堀美香
    • Organizer
      第37回日本アフェレシス学会学術大会
    • Place of Presentation
      横浜
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Proprotein convertase subtilisin/kexin 9 (PCSK9) variants in Japanese heterozygous familial hypercholesterolemia2015

    • Author(s)
      Mika Hori, Naotaka Ohta, Masatsune Ogura, Hisashi Makino, Tamiko Tamanaha, Yoshihiro Miyamoto, Mariko Harada-Shiba
    • Organizer
      第47回日本動脈硬化学会総会・学術集会
    • Place of Presentation
      仙台
    • Year and Date
      2015-07-10
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 『循環器病研究の進歩』56号2016

    • Author(s)
      堀美香、斯波真理子
    • Total Pages
      5
    • Publisher
      協和企画
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2018-03-22  

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