Local estrogen synthesis in brain metastasis of breast cancer

Research Project

Project/Area Number	15K19044
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Human pathology
Research Institution	Tohoku University
Principal Investigator	Shibahara Yukiko 東北大学, 大学病院, 医員 (30645932)
Project Period (FY)	2015-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords	乳癌 / 病理学 / 遠隔転移 / 脳転移 / エストロゲン / 転移 / 間質細胞 / アストロサイト / 癌間質
Outline of Final Research Achievements	We collected metastatic tumor tissues from breast cancer patients. Metastatic sites included brain, lung and bone. Histopathological and immunohistochemical analysis of metastatic tumors was done. We reported these results at the 104 th Annual Meeting of the Japanese Society of Pathology. Next, we did in vitro studies using rat and human astrocytes and breast cancer cells. Astrocytes and cancer cells were cocultured and analyzed for proliferation. RNA and cytokines were measured using microRNA microarray and cytokine array. Further analysis is ongoing.