| Budget Amount *help |
¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
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| Outline of Final Research Achievements |
Synovial sarcoma is an aggressive sarcoma with specific reciprocal chromosomal translocation of SS18 (also known as SYT) and SSX genes. In the present study, we aimed to detect the SS18-SSX fusion gene product in routinely processed pathological synovial sarcoma tissue section. Monoclonal antibodies to amino acids peptide, which covered the fusion region of SS18-SSX, were newly established and subsequently characterized by Enzyme-Linked ImmunoSorbent Assay, Western-immunoblotting, and Immunohistochemical staining. We generated over thirty antibodies, which immunostained SS18-SSX gene product in routinely processed formalin-fixed and paraffin-embedded tissue section; however, most antibodies exhibited the cross reactivity with SS18 and/or SSX proteins as well as SS18-SSX chimera protein. By contrast, a monoclonal antibody designated AS1SS, preferably reacted with boundary region of SS18-SSX chimera protein.
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