Pathophysiology of human glioblastoma from the viewpoint of nutrient and osmotic signals caused by tumor necrosis

• Project/Area Number: 15K19055
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Yamaguchi University
• Principal Investigator: ISHII Aya 山口大学, 医学(系)研究科(研究院), 助教 (50634747)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 病理学 / 癌 / 細胞・組織 / 脳神経疾患 / 脳腫瘍

Outline of Final Research Achievements

Glioblastoma is one of the most malignant tumors in the brain. Necrosis in the tumor tissue is characteristic of glioblastoma. In this study, we examined the functional changes of glioblastoma cells caused by osmotic pressure and nutrient shortage. The expression levels of NFAT5 protein were elevated in hyperosmolarity. In a cell line, colony-forming activity was elevated when the cells were pretreated in hyperosmolarity. The expression levels of GINS proteins were down-regulated in a shortage of glutamine or glucose.